"Liquid chromatography" Essays and Research Papers

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    The vapor pressure of a liquid is the equilibrium pressure of gas molecules from that liquid (i.e.‚ the results of evaporation) above the liquid itself. A glass of water placed in an open room will evaporate completely (and thus never reach equilibrium); however‚ if a cover is placed on the glass‚ the space above the liquid will eventually contain a constant amount of water vapor. How much water vapor is present depends on the temperature‚ but not on the amount of liquid that is present at equilibrium

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    Lab Report Purpose The purpose of this lab is to determine the identity of an unknown liquid by measuring its density and its boiling point and try to match it with those solutions given in Table 2 of experiment 2. Procedure Part A In Part A‚ The main purpose was to find the determination of the density of the unknown (j41) and by doing that we had to determine volumes of the unknown liquid (j41) using three different volumetric devices which are graduated cylinder‚ pipette and burette

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    2:05 P.M. Purpose: The purpose of the experiment is to measure the densities of liquids. Specifically‚ the two liquids that were used and measured in the research were deionized water (H2O) and alcohol (C2H5OH). Methods: Density is an intensive quantity that cannot be visually identified. Therefore‚ the most straightforward approach to this problem was to record the measurements of mass and volume. For volume of each liquid‚ the instruments used were a 50ml burette‚ a 50ml graduated cylinder and a 10ml

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    liquid crystals

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    LIQUID CRYSTALS Introduction What are Liquid crystals ? Its History & Discovery Any of various liquids in which molecules are regularly arrayed like a solid crystal along one or two dimensions‚ but are free in the other dimensions as with typical liquids. Liquid crystals often display unusual and often manipulable optical properties such as anisotropic scattering. They can be divided into two classes‚ Thermo tropic(nematic‚ chiral nematic‚ and smectic.) and Lyotropic. Transitions to thermotropic

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    COLUMN AND THIN LAYER CHROMATOGRAPHY Group 9 RAMOS‚ Sharina Joy; REYES Aina Marie; REYES Jallisa Maan; RUBIO‚ John Michael; SABINO Patricia Anne; SANTOS‚ Carlos Rafael ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components‚ the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment‚ the solid used by the students was

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    Experiment 5 Liquids and Solids Chemistry 1211 Fall 2012 Section 1: Abstract‚ Experiment and Observation Abstract: The purpose of this experiment is to determine the boiling point of a liquid and the melting point of a solid. We also were able to observe the difference between boiling and melting point Experiment and Observation: We used isopropyl alcohol and set up an experiment to observe the boiling point. We set up a second experiment using acetamide to determine

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    Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography

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    Background Information and Research Paper chromatography uses selective adsorption on a strip of paper. It is used for separating and identifying mixtures that are or can be colored‚ especially pigments. This can also be used in secondary or primary colors in ink experiments. Is it used for Contaminants in rainwater‚ Analysis of narcotics‚ and Detection of substances in urine. Purpose • The purpose of this lab is to see the separation of dye on the candy. Data and Observations Marker Color

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    In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin

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    Mobile liquid phase is always passed stationary phase during chromatography. Developed TLC plate is then observed under short wave ultraviolet light‚ where most TLC plates contain fluorescent mixed with silica gel that allow to glow green color. Compounds will absorb the UV light and appear as dark spot against the green color TLC plate. In a column chromatography‚ cotton and sand is placed in purpose of holding silica gel and prevent

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