Reetika Kashyap September 20th‚ 2012 Enzyme Lab What is an enzyme? Enzymes are specialized protein molecules simplifying most of the body’s metabolic processes such as‚ supplying energy‚ digesting foods‚ purifying your blood‚ executing the body of waste products etc. Enzymes act as catalyst by speeding up the reactions that happen in our bodies and decreasing the amount of activation energy needed to break a complex down. A reactant is any given enzymatic reaction is called a substrate for that
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higher the concentration of hydrogen peroxide the faster the reaction will occur and the more foam will be produced. Aim: The aim of this investigation is to determine how the concentration of hydrogen peroxide effects the intensity of the reaction. Variables: Independent- The concentration of hydrogen peroxide. Dependant - The intensity of the reaction. Constants – The temperature‚ the amount of liver‚ pH level‚ test time‚ Materials: Solutions of hydrogen peroxide varying in concentration 1
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Investigating the effect of different environmental factors on the reaction of liver and hydrogen peroxide Table of Contents 1 Design 3 1.1 Variables 3 1.2 Safety and Environment 3 2 Data Collection and Analysis 3 2.1 Collected Raw Data 3 2.2 Qualitative data 5 2.3 Processed Data 5 2.4 Graph on test tube 2 5 2.5 Graph on test tube 3 6 2.6 Errors 6 3. Conclusion and Evaluation 6 3.1. Conclusion 6 3.2. Evaluation 7 3.2.1. Random Errors 7 3.2.2. Systematic Errors 7 3.3. Improvements
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nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of substrate on the enzyme’s reaction. Research question: What does the changes in the amount of substrate on an enzyme’s reaction effect on? Hypothesis: I forecast that the more concentrated the hydrogen peroxide is the higher the volume of the foam is and the less concentrated the hydrogen peroxide is the lower the volume of the
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increasing the kinetic motion of molecules‚ changing the rate of collisions between them and the hydrogen bonds it’s 3D structure(D. Fraser‚ 55). There is a specific temperature for every enzyme where activity is maximized‚ and also where an enzyme becomes denatured. An enzyme becomes denatured when it is heated at extreme temperature; the excessive kinetic movement of the amino acid begins to break the hydrogen bonds holding its 3D structure together‚ typically around 70℃. Another factor that can affect
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llllllllllllllllllllllllllllllllllllllllllllllllllldfdkf Determine the presence of catalase enzyme activity using hydrogen peroxide. 1. Test for the presence of catalase in liver. A. Using a knife‚ cut a slice of liver that measures approximately 1 cm by 1 cm and place it on a slide. B. Using a micropipette‚ place a few drops of hydrogen peroxide (H2O2) on top of the liver. C. Observe the solution for a possible reaction‚ indicated by bubbling. Record the results. 2. Test for the presence of catalase in lunch meat. A. Cut a piece of roast
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Respiratory Acidosis and Alkalosis Activity 1: Normal Breathing 1. At 20 seconds‚ pH = 7.41 2. At 40 seconds‚ pH = 7.38 3. At 60 seconds‚ pH = 7.39 4. Did the pH level of the blood change at all during normal breathing? If so‚ how? Yes it did. It went down and then back up a little bit. 5. Was the pH level always within the “normal” range for the human body? yes 6. Did the PCO2 level change during the course of normal breathing? If so‚ how? No it did not Activity 2a: Hyperventilation
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what will happen in the experiment. Give your reasons and outline any assumptions that lead you to this hypothesis. I’m guessing that the liver will turn the peroxide into something else‚ or make it change. Experimental Design List the materials used in this lab‚ and the procedure you followed. Materials: Commercially available bottle of hydrogen peroxide‚ from drugstoreLiver‚ either chicken or beefAn onionA small potatoAn Apple (peeled)Wooden sticks (can be the ones used for shish kebobs)MatchesSharpie
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peroxisomes? Where do you find them? What role do they play in cellular metabolism? Explain their role in hydrogen peroxide metabolism. Peroxisomes are single membraned organelles that contain the enzyme catalase‚ which is responsible for the breakdown of hydrogen peroxide. Eukaryotic cells typically have peroxisomes present in the cell‚ however they are most commonly found in the “kidney and liver
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Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes‚ and to investigate the effect of temperature‚ surface area‚ pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1
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