tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase‚ catalase‚ lactose and sucrose fermentation‚ Kugler/iron agar‚ nitrate reduction‚ gelatin hydrolysis‚ starch hydrolysis‚ manitol salt‚ MR-VP‚ citrate‚ bile esculin‚ indole‚ urease‚ DNase‚ and coagulase. Material & Methods The tests performed on the
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Bacterial Diversity Project John FreesackSection A24 Kim Daffer‚ John Chang September 23‚ 2012 Introduction: Bacteria are everywhere. Some can be seen with the naked eye and some require a microscope but how do we distinguish one kind of bacteria from another? To answer this question‚ we were required to complete three bacterial labs that helped us to understand what microorganisms are and how to identify and classify them. Thus‚ the main purpose of this project is to identify our unknown microorganisms
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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Classifying Reactions Safety Reminder: Wear safety glasses and use ammonia in a well-ventilated area. Day 1 Materials: Part I: steel wool tweezers vinegar small jar or drinking glass water small bowl Part II: hydrogen peroxide small pieces of raw potato (yeast or beef liver may be substituted for the potato) small bowl Procedures: Part I: Reaction between iron and oxygen 1. Break off a small piece of steel wool and soak it in vinegar for at least one minute. Use tweezers to push the steel
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experiment in a well-ventilated area. Day 1 Materials: Part I: steel wool (HEB‚ Target‚ Lowe’s‚ Home Depot) You can also try SOS pads. tweezers vinegar test tube (in your kit) water petri dish (in your kit) Part II: hydrogen peroxide small pieces of raw potato (yeast or beef liver may be substituted for the potato) small bowl Procedures: Part I: Reaction between iron and oxygen 1. Break off a small piece of steel wool and soak it in vinegar for at least one minute. Use tweezers to push the steel
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produces urease to break down urea. An inoculation onto Kligler’s iron agar determines if an organism can ferment glucose and lactose‚ it also detects the production of hydrogen sulfide from the breakdown of cysteine. Our Kligler’s iron agar showed acid with gas production‚ meaning K. pneumoniae fermented both glucose and lactose. The hydrogen sulfide production was negative. A Litmus Milk test is done to determine whether the organism can ferment lactose‚ digest the milk proteins using proteases‚ cause
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difficulties because of the rare occurrence and high costs. The aim of using the microbial enzymes is to achieve this problem. Five of the more common types of microbial enzymes involved in the dairy industry involve: Rennet‚ Proteases‚ Lactase‚ Catalase‚ and Lipases. Milk contains proteins‚ especially caseins which maintain its liquid form. Proteases are enzymes that are added to milk during the process of cheese production‚ to hydrolize caseins‚ like kappa caseins‚ which stabilizes micelle formation
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
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they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area)‚ temperature on function‚ reusing the catalase‚ reaction rate of iced liver returned to room temperature‚ and effects of pH on enzyme activity‚ to see how much O2 was released by each reaction. For experiment two they tested effect of temperature on liver‚ iced had a slow increase of O2 loss‚ after a minute 18.8 ml of O2 escaped
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Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
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