FHSB1214 Unit description Biology I Year and trimester of study 2015‚ Trimester 1 Title of lab report Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition. Lecturer’s name Norkhalidah Binti Jamali Investigation of the Enzymatic Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Comments are indicated by ‘>>>’ (in detail)‚ ‘???’ (unclear) or ‘xxx’ (wrong concept) FHSB1214 Biology I (201505)-Lab Report Marking Scheme (Practical
Premium Oxygen Hydrogen peroxide Chlorine
INTRODUCTION The ever increasing population in the world today has led to the mounting of sewage‚ refuse and industrial waste everywhere. This waste has no place where it could be discarded and now it has found its way into the water bodies polluting the water and thereby creating a threat to the marine flora and fauna. In the environment‚ organisms are usually exposed not just to a single pollutant but rather to a mixture of these chemicals. Mumbai is one of the busiest metropolitans in the country
Premium Toxicology
The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
Premium Bacteria Microbiology Staining
surface‚ it induces an immune response that involves hydrogen peroxide and nitric oxide. For example‚ when it reaches the gut‚ it induces what is clinically referred to as enterocolitis‚ or infection of the digestive tract (Suzuki‚ 1994). Methicillin-resistant S. aureus (MRSA)‚ just like other S. aureus strains avoids such immune response through catalase‚ which efficiently breaks down hydrogen peroxide into water and oxygen. This activity of the catalase in the escape of the host’s immune reaction has
Premium Bacteria Staphylococcus aureus Immune system
Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
Premium Agar plate Bacteria Growth medium
Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
Premium Bacteria Microbiology Microorganism
The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
Premium Bacteria Metabolism Cellular respiration
I had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
Premium Bacillus Bacteria Enzyme
yellow. If there is only glucose fermentation‚ the slant will have a yellow butt. If there are both glucose and lactose fermentation‚ there will be a yellow slant and butt. Kliger’s Iron Agar slants is also used to check the presence of hydrogen sulfide. If hydrogen sulfide reacts with ferrous sulfate‚ the slant will have a black butt‚ and the test result is positive. Otherwise‚ if the slant has a yellow butt‚ the test result is negative. For unknown microorganism #17‚ the slant turned a pink color
Premium Enzyme Metabolism Glucose
Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
Premium Enzyme Chemical reaction Chemical equilibrium