earlier the cells were cocci with long chain arrangement. After the gram stain test I carried out a catalase test. This test was carried out to investigate if my unknown was streptococci (negative) or staphylococci (positive) and or micrococci (positive). The catalase test result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide. The next step was to examine a blood agar plate to examine the colonial morphology of hemolysis
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Introduction : Solanum nigrum Linn. commonly known as Black Nightshade is a dicot weed in the Solanaceae family. Other names of S. nigrum are European Black Nightshade or just black nightshade‚ Duscle‚ Garden Nightshade‚ Hound’s Berry‚ Petty Morel‚ Wonder Berry‚ Small-fruited black nightshade. It is a species of the Solanum genus‚ native to Eurasia and introduced in the Americas‚ Australia‚ Asia and South Africa. The plant is cultivated as a food crop‚ both for its fruit and its leaves in some
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Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
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Active transport: requires that a cell expend energy to move molecules across a membrane against the solute’s concentration gradient (the side where it is more concentrated) Feedback inhibition: Metabolic reaction is blocked by its products. A product acts as an inhibitor of one of the enzymes in the pathway to regulate metabolism. Centriole: a structure in an animal cell composed of microtubule triplets arranged in a 9+0 pattern. An animal usually has a pair of centrioles within each of its centrosomes
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temperature range and limited sample size at each group. The experiment also existed two other uncontrolled variables which are the size of liver and the amount of Hydrogen Peroxide‚ hence altering the authenticity of the practical result. At each of the five experimental group‚ there were variations amongst the amount of Hydrogen Peroxide and the size of beef liver‚ which can be a problem because in a well controlled and designed experiment‚ there is only one independent variable. These uncontrolled
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**Materials : 1) Blood agar plate . 2) Mannitol Salt agar (MSA) plate. 3) DNase agar plate . 4) Novobiocin disc . 5) Inoculating loop. 6) flame ( Bunsen burner) . 7) 1N hydrochloric acid (HCl) . 8) Two slides . 9) Plasma tube. 10) 3% Hydrogen Peroxide (H2O2) . 11) One unknown plate . 12) Crystal violet. 13) Gram’s iodine . 14) Safranin. 15) Alcohol . ** Methods : An unknown labeled with number 8 was given out by the lab instructor. The goal at this point was to determine unknown gram
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be effective as a protectant against a variety of toxic chemical agents including heavy metals (Holloway and Peterson‚ 1984). The therapeutic importance of vitamin C is immense. Vitamin C stimulate the production of enzymes super oxide dismutase‚ catalase and heat shock proteins in lymphocytes to subjects exposed to physiological oxidative stress during exercise (Khassaf et.al.‚ 2003).Presently many nutraceuticals are being used in chemotherapy. Some nutraceuticals are provided with chemotherapeutic
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produced measurable acidic byproducts? Both E. coli and S. epidermidis produced measurable acidic byproduct. H.What is the cellular role of catalase? The cellular role of a catalase is to enable the breakdown of hydrogen peroxide into water and molecular oxygen. I.Which of the organisms‚ if any‚ produced catalase? S. epidermidis is the organism that produced catalase. J.Which of the organisms‚ if any‚ produced acetoin S. epidermidis is the organism that produced acetoin.
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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GROUP No.: | DATE: 02/14/13 | | NAME: | | EXPERIMENT No.25BACILLUS: Diagnostic Tests | | ASCOLI’S THERMOPRECIPITIN TEST Purpose: used to identify anthrax bacilli in animal hides and meat. Principle: This test was designed to detect B. anthracis antigens in the tissues of animals being utilized in animal by-products and thereby to reveal when these products contained ingredients originating from animals that had died of anthrax. The thermostable antigens involved are common to other
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