Erin Arroyo Lab report June 11‚ 2013 Biology 123 Professor K Title: Scientific Investigation of the Peroxidase Enzyme & Temperature Abstract: In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature
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Wanni Lin Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are
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a scarce amount of glucose (100 mg). Lactase enzyme reaction when exposed to different pH levels The purpose of this experiment is to analyze how different pH levels affect lactase activity. The three different pH levels that we will test are: pH 4‚ pH 6‚ and pH 10. To determine if lactase was able to function and produce glucose‚ we will use glucose test strips. Table 3. Lactase functioning affected by pH
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The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The
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ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
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longest then after hyperventilation‚ and then after exercise had the biggest CO2 concentration because of how quickly it was detected. See Table 1. In the second experiment‚ the original pH of the alkaline phenol red was 4.0. After adding CO2 by exhaling‚ the color changed from a deep red to a bright red. The pH of the solution also changed; it changed to a 6.0 so it became more neutral by adding CO2 which is more basic then the alkaline phenol red. See Table 2.
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Introduction In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and
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Limiting Reagent and Percent Yield Aim To determine the limiting reagent between the reaction of lead (II) nitrate and potassium iodide. To determine the percent yield of lead (II) iodide. Date Started: 13/4/12. Finished: 19/4/12. Data collection and processing Measurements: * Amount of distilled water: 75.0ml ± 0.5ml. * Mass of watch glass: 31.65g ± 0.01g. * Mass of watch glass + potassium iodide: 32.45g ± 0.01g. * Mass of potassium iodide: 0.8g ± 0.02g. * Mass of watch
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TABLE OF CONTENT NO. | CONTENT | PAGE | 1. | Title | 2 | 2. | Theory | 2 | 3. | Introduction | 2 | 4. | Objective | 3 | 5. | Apparatus | 3 | 6. | Procedure | 4 | 7. | Result | 6 | 8. | Calculation | 10 | 9. | Discussion | 13 | 10. | Conclusion | 14 | 11. | References | 14 | TITLE: H1 – Osborne Reynolds Demonstration INTRODUCTION: Osborne Reynold’s Demonstration has been designed for students experiment on the laminar‚ transition and turbulent flow. It consists of a transparent
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