concentration of o-nitrophenol (ONP) as well as the pH of the solution tested. To begin with‚ differing concentrations of ONP were tested to find the absorbance levels of ONP. Three different pH values‚ 5‚ 6‚ and 7‚ in solution‚ were tested over a period of 8 minutes and their absorbance’s recorded. Both plots of data were then used to find at which concentrations of ONP absorption levels‚ and thus enzyme activity rates‚ were at their highest across each of the three pH solutions. Based on the results of both
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Lab Report: How Do Different pH Levels Affect The Growth of Plants Introduction: Plant growth is affected by various environmental factors‚ and pH is one of those. The pH of soil and the water that plants receive can have an impact on whether they obtain nutrients or not. Ph impacts the chemical reactions that take place within a plant‚ if the pH of the soil or water that the plant receives is too low (acidic)‚the nutrients that the plant requires may become extremely soluble to the point where
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ACIDS AND BASES The reason that acid-base reactions are so important is that many of the things you come into contact with on a daily basis are either acids or bases. Most fruits are acids‚ as are carbonated beverages‚ tea‚ and battery acid. Common household bases include baking soda‚ ammonia‚ soap‚ and antacids. What are acids and bases? There are not one but three common definitions used to describe acids and bases: 1. Arrhenius acids and bases 2. Brønsted-Lowry acids and bases 3. Lewis acids
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folding (alpha beta) · Hormones (ACTH & insulin)‚ Enzymes‚ structural pro (collagen)‚ transport (hemoglobin)‚ antibodies Enzymes – lower activation E & inc. rate of rxn / do not affect overall E · Higher temp = inc enzyme action / optimal pH = 7.2 (except pepsin & pancreas) · Competitive inhibitors compete w/ substrate for binding at ACTIVE site; can be overcome by adding more substrate; Vmax not affected · Noncompetitive inhibitors bind at allosteric site; diminishes Vmax Prokaryotes
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pH Indicators pH indicators are weak acids that exist as natural dyes and indicate the concentration of H+ (H3O+) ions in a solution via color change. A pH value is determined from the negative logarithm of this concentration and is used to indicate the acidic‚ basic‚ or neutral character of the substance you are testing. Introduction pH indicators exist as liquid dyes and dye-infused paper strips. They are added to various solutions to determine the pH values of those solutions. Whereas the liquid
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carbon dioxide concentration on blood pH. Hypothesis: If the blood CO2 levels increase‚ the pH will decrease. Materials and Procedures: Materials: Willing subject (someone complete the activities of the experiment) Timer (with a second hand) Glass Urinary and Salivary pH (litmus) paper Drinking straw Procedures: 1. Measure 2 ounces of water in a glass. Obtain the pH of the water in the glass. Record the water pH prior to experiment.
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Understanding the functions of the body systems associated with energy metabolism Energy is used for many of the bodily functions including the ability to ventilate‚ respire‚ move‚ grow and repair. The first law of thermo dynamics states that energy can be transformed from one state to another but cannot be lost. Energy comes in many forms‚ some of these are as follows:- Light Sound Kinetic Heat Chemical In accordance with the First Law an example of how energy can transform is when the sun creates
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Figure 1: Titration curve of 0.160 grams of an unknown diprotic acid that was dissociated in distilled water. Shown is the pH versus the volume in milliliters of 0.1 M NaOH‚ a strong base‚ added to the solution. The initial pH reading of the solution was a pH of 2.60. Although the pH of the ½ equivalence point was unknown‚ it could be estimated by halving the volume of NaOH used at the first equivalence point. At the first equivalence point‚ 13.63 milliliters of NaOH had been added to the unknown
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Buffers CALCULATIONS Table A. pH Measurement using pH meter Calculated pH Solution 1 – HoAc 0.10 M CH3COOH CH3COOH + H2O ⇌ CH3COO- + H3O+ i 0.10 ø ø c -x +x +x e 0.10 – x x x Ka = H3O+[CH3COO-]CH3COOH = x20.10 – x = 1.8 x 10-5 x = 1.33 x 10-3 M pH = -log [1.33 x 10-3] pH = 2.88 Solution 2 – HoAc – OAc na
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The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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