CHE 107 24 June 2013 Separation of a Mixture of Solids Step 1: Separating out the Iron Mass of weighing dish: 0.7g Mass of weighing dish and mixture: 5.4g Net mass of the original mixture: 4.7g Mass of 10-cm square paper: 0.8g Mass of 10-cm square paper and iron fillings: 2.0g Net mass of iron filings: 1.2g Step 2: Separating out the Sand Mass of weighing dish: 0.7g Mass of weighing dish and dry sand: 1.7g Net mass of the dry sand: 1.0g Step 3: Separating out the Benzoic
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Gas Chromatography Analysis of Product Mixtures Gas Chromatography Guidelines‚ Handout. Introduction Gas chromatography is a technique used to analyze chemical compounds that can be vaporized and separated in a gas phase column. Once separated‚ the analyzed substance is passed through a detector and data is obtained. The samples that we are going to analyze are: the EtOAc from Simple distillation‚ the Fraction 1‚ Fraction 2‚ and Fraction 3 from the Fractional Distillation. Experimental Procedure
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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The Separation and Purification of Organic Compounds Sunshine Marie Introduction Each year‚ many thousands of new or unknown organic compounds are prepared or extracted from natural sources such as plants‚ fungi and animals. In order to identify an unknown organic compound or the components of a mixture of organic compounds‚ it is necessary firstly to separate and purify the compounds. At any given temperature‚ a certain number of molecules of a substance in the liquid state have sufficient
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LAB 4 – SEPARATION OF A MIXTURE OF SOLIDS Purpose: To determine and execute the separation of mixture of solids through different means. Examples are magnetisms‚ evaporation‚ and filtration. Observations and Experimental Data: Data Table 1: Experiment Data Grams Percent of mixture Iron filings 1.4 g 31.1 % Sand 1.3g 28.9 % Table salt 1.0g 22.2% Benzoic acid .8g 17.8 % Total 4.5g 100% Questions: A. How did your proposed procedures or flow charts
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Quantitative Separation of a Mixture Lab Pre-Lab Questions: 1. Define the following: Physical Change Heterogeneous Mixture Sublimation Filtrate Residue 2. List two physical separation methods. 3. List the formula for determining percent composition by mass. Objectives: 1. to separate a three component mixture and 2. determine the percent composition by mass of each component in the mixture. Materials: Ammonium chloride (NH4Cl)‚ Sodium chloride (NaCl also known as
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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Experiment 1 Separation of Mixtures Summary of Principles: Most of the materials we encounter in everyday life are not pure substances. Many materials such as sand are formed from physical combination of various substances‚ and are called mixtures. Mixtures can be classified into homogeneous and heterogeneous. The purpose of this lab is to learn how to separate individual components from a mixture through decantation‚ filtration‚ distillation‚ and crystallization. Pre-Lab Questions: Attached
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materials we use today are actually mixtures of pure substances. The separation of these materials into their pure substances can be quite challenging. When we are discussing separations of a mixture that contains pure substances‚ it all depends on whether you can separate the substances by physical means. That means‚ utilizing each substances physical properties such as boiling point‚ melting point‚ and magnetism. Separation of a Mixture of Solids Lab Report Assistant
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Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
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