COLUMN AND THIN LAYER CHROMATOGRAPHY Group 9 RAMOS‚ Sharina Joy; REYES Aina Marie; REYES Jallisa Maan; RUBIO‚ John Michael; SABINO Patricia Anne; SANTOS‚ Carlos Rafael ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components‚ the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment‚ the solid used by the students was
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It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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Analysis and Separation of Organic Acids in White Wine Using High Performance Liquid Chromatography Atis‚ Arnelson Arwin G. and Gaitos‚ Gerald M. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Performed: December 9‚ 2011 Submitted: December 15‚ 2011 RESULTS AND DISCUSSION solutions containing increasing amounts of the organic acid. These solutions were contained in flasks numbered 5‚ 6‚ 7‚ 8‚ and 9. (Refer to
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Ion Exchange Chromatography Week 10 TAG Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column‚ load your sample and collect the hydronium ion released. 1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the
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Black Garlic Omelet and Spinach Waking up in the morning and eating a delicious cheesy omelet sounds like a perfect morning breakfast. And with this wonderful and healthy omelet recipe‚ you will be packed with energy to last the whole day! Yields: 3 Servings Ingredients: 3 tbsp. of Cheddar Cheese 3 Eggs 1 clove of Black Garlic 3 tbsp. of Parmesan Cheese ¼ cup of Tomatoes ¼ cup of Green Onion A pinch of salt A pinch of Pepper 1 cup of Spinach Some Olive Oil/ Cooking Spray Method of Preparation:
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ANALYSIS OF MOUTHWASH Background Information: Commercial mouthwashes are mixtures of water‚ alcohol‚ dyes‚ flavorings‚ and other compounds. In this experiment‚ you will use gas chromatography to determine the alcohol content of the mouthwash. Since the volumes of alcohol and water are not additive when mixed‚ a calibration curve must be used. It will provide the correction factor needed for alcohol-water mixtures. The calibration curve will be prepared by placing a known amount of alcohol
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Discussion Using the Thin Layer Chromatography experiment‚ caffeine was found to be the most polar due to the fact that it stayed closer to the stationary phase. Caffeine contains four amine groups that are extremely polar as a result of the hydrogen bond and amide functional group. Acetaminophen was found to be the second most polar analgesic drug tested. Acetaminophen contains a polar alcohol group on one side and amide group on the other but also includes non-polar functional groups that consisted
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of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach. In the second lab period
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A pigment is a natural coloring matter that usually comes from a plant or animal. Today‚ there are synthetic pigments with the advancement of technology‚ but during the Italian Renaissance they did not have these luxuries. They had to obtain their pigment naturally. There were different classifications of pigment‚ each producing a different desired color. The material that contained the desired pigment had to be grinded very finely‚ and then the pigment would need to be added to a base to create
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RESPIRATORY PIGMENTS Colored‚ metal-containing proteins that combine reversibly with oxygen‚ found in the body fluids or tissues of multi-cellular invertebrate animals and microorganisms. The role of these pigments is primarily to aid in the transport of molecular oxygen. Thus they are distinguished from respiratory enzymes‚ which are concerned with the metabolic consumption of oxygen. Four distinctly colored groups of respiratory pigments exist among invertebrates: hemoglobins (purple‚ become orange-red
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