was made up in each case up to 10mL with methanol to produce the concentrations 1‚ 2‚ 3‚ 4‚ 5‚ 6‚ 7‚ 8‚ 9 and 10 g/mL. Absorbances of these solutions were recorded at max 405.9 nm against methanol as blank using Shimadzu (1601) UV/Visible spectrophotometer‚ which is recorded in table 5.4. Standard curve is presented in figure
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in this case is whether that expansive construction trenches on the federal constitutional rights of the Boy Scouts of America (BSA).” The majority holds that New Jersey’s law violates BSA’s right to associate and its right to free speech. But that law does not “impose any serious burdens” on BSA’s “collective effort on behalf of its shared goals‚”nor does it force BSA to communicate any message that it does not wish to endorse. New Jersey’s law‚ therefore‚ abridges no constitutional right of the
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002M FeCl3 | 0.2M KSCN | 0.1 M HCl | 0 | 3 | 11 | 2.1 | 3 | 8.9 | 2.3 | 3 | 8.7 | 2.5 | 3 | 8.5 | 3 | 3 | 8 | 4 | 3 | 7 | The experiment determined the equilibrium constant for the formation of the FeSCN2+ complex. Using a spectrophotometer‚ the absorbance of FeCl3‚ KSCN and HCl standard solutions of known concentration was measured and graphed to determine the absorptivity coefficient. This coefficient was then used to calculate the actual concentrations of the FeSCN2+ complex
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Title: Bradford Assay: Creating a Standard Curve and Determining Unknown Protein Concentrations Introduction: In biochemistry the ability to determine the quantity of a certain substance in a solution is extremely important. Often protein concentration within a biological organism is essential in determining the function or condition of that organism. If the protein concentration in an environment is increased or decreased it can have detrimental side effects. If there is a decrease in hemoglobin
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Plotting the Absorption Spectrum of Lettuce Leaf Extract November 14‚ 2012 Aim: To see how chlorophyll pigments absorb electromagnetic radiation at different wavelengths‚ with the use of a spectrophotometer to vary the wavelength of incident light‚ and then plot an absorption spectrum. Hypothesis: The chlorophyll pigments of the spinach leaf’s chlorophyll extract will absorb more electromagnetic radiation at wavelengths which correspond to the wavelengths of colors other than green‚ as chlorophyll
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As you can see in table 1‚ the percent bound of BSA-PR at pH 4 (61.543%) was highest compare to other pH. pH 6 has the percent bound of 3.569%‚ pH 8 has the percent bound of 2.330%‚ and pH 10 has the percent bound of 2.309%. pH 6‚ 8‚ and 10 do not have an optimal binding for bovine serum albumin and
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AIM: The aim of the experiment was to determine the enthalpy (ΔH)‚ entropy (ΔS) and Gibbs free energy (ΔG) for the Rhodamine β-Lactone Equilibrium. To accomplish this‚ a manual spectrophotometer was used to determine the maximum absorbance of a sample of Rhodamine β-Lactone. The absorbance of the sample was then measured over a range of temperatures from which the equilibrium constant (K)‚ enthalpy (ΔH)‚ entropy (ΔS) and Gibbs free energy were then calculated. INTRODUCTION: The xanthene
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ell SOTM LAB: B14 12/99 I. TEACHER NOTES & GUIDELINES Title of Lab: Effects of Temperature and Solvents on the Cell Membrane Developers of Lab: Adapted by James Kirby JD726‚ Jennifer Mortellaro JD449‚ and James Prockup JD575 from a publication by the Department of Biological Sciences at Western Michigan University‚ Kalamazoo‚ MI Overview of Lab: Description: The purpose of this lab is to illustrate the effects that temperature and solvents have on the cell
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HAEMATOLOGY 1 MEDS 1084/1083 SHORT REPORT Use of spectrophotometric techniques to determine the optical density or absorbance for the sample with different dilution factors NAME & STUDENT ID: MANMINDER KAUR COURSE: Haematology 1 COORDINATOR: Genia Burchall PRACTICAL DATE: 26th July‚ 2012 DUE DATE: 10th August‚ 2012 ABSTRACT: This experiment aimed at determining the optical density in terms of absorbance of a solution using the different dilution factors to obtain a standard
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prepared on dealing with any potential new situations arising. If they are against the discriminatory policy of the Boy Scouts I find that they need to communicate it in the best way possible. They need to express how much they value and respect the BSA and point out all the good things they do. In the end they can mention how disheartened they are to have to pull their funding because of antidiscrimination policies being in
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