The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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Applying Bovine serum albumin (BSA) (up to 3%) in the rung buffer could be useful. It is can be loaded into the running buffer and run with the target analytes while a interaction analysis of BSA binding on SPR sensor chip is needed in order to eliminate the signal generated from BSA binding at the final calculation. BSA is the most preferred because it can efficiently block the non-specific binding and no significant harmful
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Materials ● ● ● ● ● ● ● Substrate solution Cuvette Spectrophotometer Timer Enzyme solutions Pipette for transferring fluids Thermometer Methods ● There is a choice of six different enzyme & substrate combinations in which the temperature will vary at which the reaction as well as the enzyme & substrate concentrations. pH: 1. A SUbstrate B/ Enzyme B was selected along with setting the wavelength to substrate vial/ 680 & the spectrophotometer to read light absorption. 2. The temperature was set to 25 degrees & set to different
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clumps of the potato extract formed on the bottom of the test tube and interfered with the spectrophotometer. Whereas‚ the lower bathed temperatures had icicles‚ which reflected the light back of the spectrophotometer. It was concluded that the murky colored tubes shouldn’t be included because it would be a systematic error due to the excess potato extract and murkiness interfered with the spectrophotometer. Moreover‚ in Figure 3A and 3B‚ hydroquinone is the enzyme that produces the dark color. The
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drink is conducted to determine concentration of the caffeine and the benzoic acid mountain dew. The UV/Vis spectrometer is used and the ultraviolet absorbance is chosen to conduct the spectrophotometric analysis in this experiment. The UV/Vis spectrophotometer work by passing the light by strong lamp through a monochromator‚ which split the light into its component colours using a gratings. Then‚ the desired wavelength of light beam is direct through the sample compartment which the cuvette is placed
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Quantifying the COX1 Gene within the Mitochondrial DNA of a Potato Introduction Respiration is a very important process for every living organism. While it is typically thought of as breathing in oxygen‚ and exhaling carbon dioxide‚ like all things‚ it must take place at the cellular level. The electron transport chain is responsible for cellular respiration. The process uses four complexes; the fourth is cytochrome c oxidase. Cytochrome C oxidase is responsible for the reduction of oxygen to water
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Introduction Photosynthesis takes place in the chloroplast and is the use of sunlight energy for cell processes. It happens through a number of chemical reactions and the transfer of electrons. It is used as the source of energy (ATP) for plants. The reaction equation for photosynthesis is H2O + CO2 + (Light) (CH2O)N + CO2 In the Hill reaction‚ Robert Hill showed how chloroplasts in water can still function if there is light and an electron acceptor to release oxygen. He proved that the oxygen
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Solution ‘Concentration’ Using A Spectrophotometer” lab was to use a spectrophotometer to find the relationship of concentration and absorbance obeying the Beer-Lambert law‚ which states concentration and absorbance are directly related‚ to then further determine the concentration of three unknown solutions. With the assumption that the solutions obey the Beer-Lambert law it is predicted that as concentration increases‚ absorbance will increase as well. A spectrophotometer is a device that uses a light
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color change (Stegenga 24-8). In class‚ we observed this happening using a lamp and a spectrophotometer to measure the amount of light being transmitted through a test tube. Chloroplasts were placed into test tubes along with water‚ DPIP‚ and a buffer to neutralize the pH of the solution‚ and then placed under a lamp with a standard 40-watt light bulb. One test tube was used to calibrate the spectrophotometer so that each transmittance reading would be
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Colorimetry Introduction Absorption Spectroscopic methods of analysis rank among the most widespread and powerful tools for quantitative analysis. The use of a spectrophotometer to determine the extent of absorption of various wavelengths of visible light by a given solution is commonly known as colorimetry. This method is used to determine concentrations of various chemicals which can give colours either directly or after addition of some other chemicals. As stated by the Beer-Lambert Law
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