Lesson Learning Outcomes Upon completion of this lecture‚ students should be able to: understand the structures and properties of amino acids know the groups of amino acid What are amino acids? Amino acids are molecules that when combined with each other proteins. Amino acids contain a central tetrahedral carbon atom (α-carbon) amine group‚ carboxyl group‚ R-side chain The R-side chain determines the different amino acids There are 20 common amino acids Amino acids can join via peptide
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reactors were taken for analysis. 120 121 2.3. Analysis methods 122 A high performance liquid chromatography (LC-20A‚ Shimazu) coupled with a C18 analytical column (4.6 123 mm × 150mm‚ 5 μm particle size) was used to determine the concentration of
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in the mixtures was provided along with the boiling points. Experimental Procedure: The simple distillation apparatus was constructed as instructed (Figure 1). A ~5mL vial was equipped with a stillhead‚ air and water-cooled condenser (thick column)‚ claisen head‚ Hickman distillation head‚ thermometer‚ and thermometer adapter. The equipments were tightly clipped to prevent vapor from escaping apparatus. A thermometer was placed into stillhead with the support of thermometer adapter. The thermometer
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To begin the experiment‚ the reaction apparatus was assembled (as shown in Figure 1 below from the lab manual) consisting of a 3.0 mL conical vial charged with p-cresol (80uL from Eppendorf pipette)‚ 25% aqueous NaOH (130uL)‚ and a spin vane. The solution was mixed thoroughly and tetrabutylammonium bromide (9mg) was added along with n-propyl iodide (75uL) and it was equipped with a flask with a water reflux condenser. The solution was heated (95-100°C) while vigorously stirring it. After 60 minutes
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3.13 Test for Saponin glycosides 2g of dried plant powder add 5ml of distill water shake vigorously. Persistent of foam indicate the presence of saponin glycosides. 3.14 Test for Steroids (Salkowski’s test) 5ml of test solution add with 2ml of Chloroform and then Conc. H2SO4 sides of the test tubes. The appearance of color at the interface indicates the presence of steroids. 3.15 Experimental Procedure for preliminary Phytochemical studies (Brindha et al.‚ 1981) S. No Name of the Test
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...................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ............................................................ 25 1. Chromatography ................................................................... 25 2. Electrophoresis ..................................................................... 53 III. Immunoassays .............................................................
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column was clamped in a vertical potion to the stand. 8mL of eluent was added to the column and the silica gel was mixed with the remainder of the eluent. The mixture was carefully poured into the column. More eluent was added to the beaker until all the silica could be poured into the column. The eluent was drained and a 1cm layer of sand was added to the top of the silica. More eluent was added and drained until it was level with the top of the sand. Extraction of pigments 3-4 baby spinach
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pooled and concentrated in vacuum at 40°C using a rotary evaporator. The crude extract was then diluted in minimum amount of chloroform (2ml) and citrinin was estimated by thin layer chromatography (TLC) according to Razak et al.[16] Subsets of citrinin obtained were estimated by High performance Liquid Chromatography (HPLC). The control contained PDB broth and 10 μl of fungal suspension. Briefly‚ different volumes (1 to 5 µl) of sample extracts were applied to precoated
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ZINC AND NICKEL CONCENTRATION BY (1) ION-EXCHANGE CHROMATOGRAPHY FOLLOWED BY CHELOMETRIC TITRATION‚ AND BY (2) ATOMIC ABSORPTION SPECTROSCOPY OF THE MIXTURE Experiment 4 Dates of Experiment: 10/14/08 through 10/30/08 Date of Report: 11/7/08 Chem 2262L � I. Introduction In this experiment‚ the zinc and nickel contents of unknowns were tested using two methods. In the first method‚ nickel and zinc were separated through ion-exchange chromatography and analyzed through chelometric titration. In
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some reagents that will cause the ferrocene to add either one acetyl group to an aromatic ring or add two acetyl groups to each of the aromatic rings. In order to determine how well this process had worked we employed: IR spectra analysis‚ column chromatography‚ and a little TLC. This experiment is relevant in today’s highly industrialized world. By utilizing many of the techniques we employ in this lab‚ a company can synthesize new types of materials or composites that could revolutionize an industry
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