In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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Column and thin layer chromatography of plant pigments CHEMISTRY 201L EXPT 04 PAGE 01 - 12 Noel Angelo P. Kalacas*‚ Hanna Mae Laluces‚ Ina Bianca Lanuza Department of Chemistry‚ College of Science *Corresponding author; e-mail: knight_BeNcH66@yahoo.com Abstract Chromatography is a powerful technique for separating and/or identifying the components in a mixture. There are different types of chromatography and each has its own strengths and weaknesses. In this experiment‚ pigments of the
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corresponding anthocyanidins using paper chromatography‚ and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g‚ followed by blackberries at 1.19 x 10-6 g‚ finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins‚ since it contained the most streaks on the chromatography paper. The main conclusion that can be
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5.05 Ink Chromatography Basic Lab Components Background Information and Research: 1. Paper chromatography type of method that is used to separate mixtures of substances from a solution. 2. There are many uses for paper chromatography‚ especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to identify clues at a crime scene such as blood‚ ink‚ or drugs. However‚ the more common use is to
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Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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Results and Discussion 2.5.1. Morphological characterization of hollow fiber capillaries from FESEM images The longitudinal view of the hollow fiber is presented in Fig.3 (a). The outer surface was further magnified to look into the pore details in 5KX and 25 KX magnification‚ are shown in Fig.3 (b) and (c).The image of the hollow surface revealing the cut surface showing the inner layer of the hollow fiber are shown in Fig.3 (d). The fine structures of the fibers ‚ shown in Fig.3 (e) and (f) resembles
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6. FIRST DERIVATIVE ZERO CROSSING METHOD 6.1. ABSTRACT: Simple‚ specific‚ accurate and precise 1st order derivative zero-crossing spectroscopic method was developed for simultaneous determination of PHENYLEPHRINE and EBASTINE from their binary mixture. In this 1st derivative zero-crossing spectroscopic method the amplitudes of the 1st order derivative of the spectra of the binary mixture containing PHE and EBS were measured at 252.2 nm (zero crossing of EBS) for determination of PHE and at 274.8
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. Yes‚ comparably to TLC plate 2 and 3. The benzophenone is more neutral but as the same time its nonpolar‚ being that Silica gel in TLC 2 was polar and the solvent ethyl acetate-hexane is non-polar it moved relatively with the solvent. The stationary phase in TLC 3‚ alumina is non polar and the solvent ethyl acetate is polar so from the ideal that polar does not attract non-polar benzophenone stick stronger to alumina non-polar rather than moving up with polar solvent ethyl acetate as it did in
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Name: __________________________ Date: _____________ 1.|Complete the table and use the result to estimate the limit. x|||||||f(x)|||||||| A)|–0.076923| B)|0.423077| C)|0.298077| D)|0.548077| E)|–0.451923| 2.|Determine the following limit. (Hint: Use the graph of the function.)| A)|6| B)|5| C)|1| D)|4| E)|does not exist| 3.|A graph of is shown and a c-value is given. For this problem‚ use the graph to find .| A)|0| B)|–10| C)|–5| D)|15| E)|does not exist| 4.|Use
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Kayla Adkins Forensic Chemistry Exam 2 I have been honest and observed no dishonesty. 1. In specific detail‚ describe a published procedure for the definitive identification of Heroin utilizing gas chromatography-mass spectroscopy (GCMS). Heroin is a white crystalline powder with no odor and a bitter taste. It is a derivative of morphine in that the –OH (hydroxyl group) is replaced by an acetyl group‚ -OCOCH3 and has a molecular formula is C21H23NO5 with a molecular weight of 369.42
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