assess the concentration of the protein because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the colour‚ and therefore the absorption‚ is directly proportional to the protein concentration. Q 2. What is the experimental reason for constructing a standard curve? Multiple samples with known properties can be measured and graphed‚ which then enables the same properties to be determined for the unknown samples‚ in this case the concentration‚ by interpolating
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9 4 5 6 6.00 D 4 4 3 3 3.50 E 3 4 1 3 2.75 Table two: Calculation of cell count (ml-1) Tube Average Count for four (4x4 grids) Average Count ml-1 (no. X 104) Final Dilution factor Original cell concentration A 32.75 327500 × 4 131 × 104 B 10.25 102500 × 8 82 × 104 C 6.00 60000 × 16 96 × 104 D 3.50 35000 × 32 112 × 104 E 2.75 27500 × 68 187 × 104 Table Three: Percentage of cell viability (%) Tube Average viable cell count
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Practical Work Nº2: “Different methods to control osmosis” Aim: observe and test the process of osmosis through different kinds of methods: the weigh (potato)‚ the density (beetroot) and under the light microscope (onion). Hypothesis: according to the encyclopedia definition osmosis is the diffusion of a liquid (most often assumed to be water‚ but it can be any liquid solvent) through a partially-permeable membrane from a region of high solvent potential to a region of low solvent potential. Thus
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Concentration Problem Sets Do the following problems in your lab notebook. Be sure to specify the knowns & unknowns‚ the formulas used‚ show your work with labeled units and round you’re answers to the appropriate significant figures. 15-1 Molarity Problems: (M = moles/ liter ) 1. What is the molar concentration of a 415 ml solution containing 0.745 moles of HCl? (A: 1.80 M HCl) 2. What is the molar concentration of an acetic acid (CH3COOH) solution containing 3.21 moles in 4.50
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Water Concentration in Potato Cells Formal Lab Report Purpose: The purpose of this lab is to determine the concentration of water in potato cells. Method: To complete this task‚ we will first soak our potato cells in different concentrations of sugar solutions. Before we actually soak them‚ we will need to know the initial mass of the potato cores. After soaking‚ we will need to know the mass of the final core. We will use the data we collect to find the percent change in mass of the cores
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OSMOSIS LAB REPORT INTRODUCTION Osmosis is a special type of diffusion where water molecules move down a concentration gradient across a cell membrane. The solute (dissolved substance) concentration affects the rate of osmosis causing it either to speed the process up or slow it down. Based on this‚ how does different concentrations of sucrose affect the rate of osmosis? If sucrose concentration increases in the selectivity-permeable baggies‚ then the rate of osmosis will increase. MATERIALS AND
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Chemistry Unit 2 Area of Study: 1: Water Chapter 11: Measuring solubility Measuring Solubility Solubility: the maximum amount of that substance that can be dissolved in a that temperature Saturated solution: a solution which no more solute can be dissolved at that temperature Measuring solubility Determine the maximum mass of solute that can be dissolved in 100 grams of solvent at a particular temperature Worked Example A maximum of a 6g of solute can be dissolved in 20g of water at 20ºC
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You correctly answered: d. glucose and albumin are both too large to pass. The rate of diffusion for urea You correctly answered: b. is slower than that for sodium because urea is a larger molecule. Experiment Data: Solute MWCO Solute Concentration Average Diffusion Rate Na+ Cl- 20 9.00 0.0000 Urea 20 9.00 0.0000 Na+ Cl- 50 9.00 0.0150 Na+ Cl- 50 18.00
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1. Diffusion and Osmosis‚ June 4‚ 2013 2. Purpose: The purpose of this lab is to help give visual understanding of diffusion which is a solution of high concentration spontaneously (no energy required) moving to an area of low concentration. Also taking a look at osmosis‚ which is the movement of chemicals across the cell membrane. Osmosis requires some type of energy to be put in for this to happen. After these experiments we should have a better idea and visualization of how chemicals transfer
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2. Explain your graph : 3. Define simple diffusion: 4. Define osmosis: Osmosis can be defined as the movement of solvent molecules across a semipermeable or selectively permeable membrane ‚from a region of lower concentration of the solute to that of a higher concentration till equilibrium is attained. 5. What is the change in the level of molasses in 10 minutes? 6. What is the change in the level of molasses in 30 minutes? 7. What force is responsible for the movement of substances
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