Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that‚ specific biochemical tests were performed for gram positive‚ since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak‚ Blood Agar streak‚ Catalase test
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patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain + Fluid Thioglycollate Strict aerobe Catalase + Nitrate Reduction + Growth 5% NaCl - Growth 7.5% NaCl + Gelatin Hydrolysis - Glucose Fermentation + My organism appeared to consist of gram-positive cocci‚ making it a member of Group 17 in Bergy’s
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Pseudomonas aeruginosa Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had‚ was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane‚ periplasmic space‚ peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any
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aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant‚ the P. aeruginosa colonies had a filiform appearance on the edges. I think we correctly identified our unknown as P. aeruginosa because we performed several different tests‚ eleven of which helped us identify our organism on the Gram negative chart that was in our lab
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number 12 was picked. According to the procedures that we have been learned in class‚ our performances were running properly. More than 20 kinds of experiments were performed to identify unknown bacterium. The first test is gram stain. The result is pink rod which means gram negative rod shape cells. Also‚ in nutrient broth tube‚ it is observed to growth bacterium. To determine the production of protease‚ we do the gelatin stab. The result of this experiment is negative which has solid texture. The
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MOLEFHI | Identification of entrobacteriaceae | Escherichia coli cause 99% of URINARY TRACT INFECTION. | DAY 2 GRAM STAINNING Materials * Two slides * Saline water * Culture organisms * Wire loop * Gentin violet * Iodine * Safradin * 95% alcohol Procedure * Prepare slide smears of both cultured organisms and heat fix them. * Gram stain with first using Gentin violet as primary stain then‚ * Iodine as a mordent and decolourise with 95% alcohol
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samples to controls. Both leaves were boiled in water then placed in hot ethyl alcohol. After adding the iodine solution‚ the dark leaf was brown in the middle and the outer edges were bluish black. The light leaf was brown all over. 3. State your conclusions in the form of a hypothesis which addresses the metabolic pathways that constitute plant photosynthesis.
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The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast
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3.1. Isolation and identification of Bacterial isolates An enrichment culture technique was used for the isolation of bacteria responsible for biodegradation of phorate in soil. Screening of these bacterial species for phorate degradation in liquid cultures in our previous study (Jariyal et al.‚ 2014)‚ resulted in identification of bacterial species B. aerophilus strain Imbl 4.1 ‚ Brevibacterium frigoritolerans strain Imbl 2.1 and Pseudomonas fulva strain Imbl 5.1. However‚ these bacterial species
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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