CHAPTER I INTRODUCTION Background of the Study Mangrove swamps are forested intertidal ecosystems that occupy sediment-rich sheltered tropical coastal environments. By trapping and stabilizing fine sediments‚ mangroves control the quality of marine coastal waters. Aside from maintaining coastal food webs and populations of animals‚ mangroves have an important role in pollution control through their absorptive capacity for organic pollutants and nutrients‚ and they play an important role in storm
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Russel Roberson‚ Franc¸ois Elvinger. 2001 “Use of eosin methylene blue agar to differentiate Escherichia coli from othergram-negative mastitis pathogens”. J Vet Diagn Invest 13:273–275 3. Hiroshi Fujikawa‚ 1994. “Diversity of the growth patterns of Bacillus subtilis colonies on agar plates”. FEMS Microbiology Ecology 13: 159-167 4. Anna Csillag‚ 1972. “Appearance of acid-fast rods in cultures of mycococcus luteus”. Tubercle and Lung Disease 53: 221-225 5. James A. Bastock‚ Michelle Webb and Jane A.
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Plasmodium‚ Trypanosoma Algae: Diatoms‚ Spirogyra‚ Volvox Helminths: Tapeworm‚ fluke Fungi: Methylene blue stain of yeast cells (Saccharomyces)‚ sporangiospores of Rhizopus‚ conidiospores of Penicillium Staining Labs (3-6‚ 3-7‚ 3-8‚ and 3-9) – Identify and correctly interpret the following: Lab 3-6: Gram stain Lab 3-7: Acid Fast stain Lab 3-8: Capsule stain Lab 3-9: Endospore stain Metabolic Tests: Lab 5-1: O-F Glucose (aerobic versus fermentative organisms) Lab 5-2: Phenol Red broth for sugar
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with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey
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common between Archaea and Bacteria. In terms of their cell structures‚ Archaea are indistinguishable from Gram-positive bacteria. Within prokaryotes‚ only these two groups of organisms are bounded by a single unit lipid membrane‚ and they generally contain a thick sacculus of varying chemical composition. Some Archaea also show positive Gram staining and a few of them‚ similar to certain Gram-positive bacteria‚ do not contain any cell wall (Bacterial‚ 2006). The similarity between Archaea and Bacteria
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Trypanosoma Protozon-Trypanosoma is a genus of kinetoplastids (class Kinetoplastida)‚ a monophyletic group of unicellular parasitic flagellate protozoa. E Coli-Escherichia coli is a Gram-negative gammaproteobacteria commonly found in the lower intestine of warm-blooded organisms Staphylococcus -Staphylococcus is a genus of Gram-positive bacteria. Under the microscope‚ they appear round‚ and form in grape-like clusters. The Staphylococcus genus includes at least 40 species. Giardia Protozon-Giardia lamblia
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leprae III. Description of the Bacteria Mycobacterium leprae‚ the bacteria which causes leprosy‚ is a rod-like bacillus with parallel sides and rounded ends measuring 2-7 micro-millimeters in length and 0.3-0.4 micro-millimeters in width. It can only be seen under the electron microscope when stained. It is acid-fast and alcohol-fast‚ meaning not decolorized by acid or alcohol after staining‚ as most bacteria retain dye after an acid or alcohol rinse. It occurs in large numbers in the lesions of Lepromatous
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master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with me on the journey to the unknown. As I recall from earlier that semester‚ the first test that needed to be performed was the Gram stain. The Gram stain is a staining technique that aids in distinguishing cell wall characteristics and the cellular morphology. Bacteria will stain Gram positive‚ which is a purple color‚ or Gram negative‚ which is a pink
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procedure called gram stain is used in the identification of bacteria. Bacteria can be gram-positive‚ gram-negative‚ or gram-variable. Most bacteria however‚ are usually gram-positive or gram-negative. Gram-positive and gram-negative bacteria react differently to staining because of the differences in their cell walls. Bacterial cell walls contain a polymer called peptidoglycan. Gram-positive bacteria have more of this polymer than gram-negative bacteria. They are also less complex than gram-negative
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Abstract: It is important to be able to identify pathogenic bacteria that may be causing harm. Tomato crops can be affected by several different pathogenic bacteria. By using Koch’s postulates‚ it was determined that Pseudomonas syringae was the bacteria causing rot. There are four criteria that must be met when using Koch’s postulates. They are that the organism must be fund in all infected‚ the organism must be isolated in pure culture then once reinnoculated in a healthy host‚ must cause the
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