desired specimen. It also took practice in adjusting the light source so as to have the proper contrast and brightness. In the end the practice using the prepared slides allowed for clear observation of the various bacterial cell shapes such as cocci‚ bacillus‚ and spirillium. These shapes were more easily identified in the prepared slides and this knowledge was used to view similar structures in the fresh yogurt slide. Finally‚ analysis of a blood smear was interesting because it was initially very hard
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microbiology lab techniques including an API test‚ an oxidase test‚ a gram stain‚ a hanging drop slide‚ and morphology identification. The unknown bacterium‚ which was contaminated with Serratia marcescens‚ was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria‚ had the appearance of circular form‚ convex elevation‚ entire margin‚ elongated cocci. The tests than showed that the bacteria was gram-negative‚ non-motile‚ and was identified as a Salmonella species
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Hartpury College Fdsc Equine Science Animal Microbiology Diagnosis and treatment of disease UIN XGJ-10-1 Amanda Finch Date Set: 20th September 2010 Date due: 17th November 2010 Contents page Page Title page i List of contents
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This particular type of agar only allows for the growth of gram negative rods. In performing this particular test‚ distinguishing factors between lactose fermenters and non-lactose fermenters can be shown. If the organism applied to the agar produces orange colonies‚ and if the area around the bacteria turns orange
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preparation microscopy 4 Gram stain 4 Culturing 4 MATERIALS TO BE USED 5 Microorganisms known to cause diarrhoea 5 Other material 5 Wet preparation microscopy 5 Gram stain 6 Culture 6 Biochemical tests 6 Glass ware 7 ETHICAL CONSIDERATIONS 7 SAMPLING COLLECTION 7 SAMPLE ANALYSIS METHODS 8 EVALUATION OF RESULTS 8 DISSEMINATION OF RESEARCH RESULTS 9 WORK AND TIME SCHEDULE 9 FINANCE BUDGET 9 Other requirements 9 Wet preparation microscopy 9 Gram stain 10 Culture
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known that were mentioned above. First it was the Gram staining procedure used to categorize bacteria in two groups‚ gram positive and gram negative. Gram staining is one of the most useful test used in the clinical setting to identify bacterial colonies due to their broad staining spectrum. (Black‚ 2008‚ pp. 70-71) The basis of the Gram stain is that gram positive bacteria retains the color of the primary dye‚ the crystal violet‚ whereas the gram negative bacteria loses the primary dye once its
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Microbiology: study of small living things study of bacteria: bacteriology study of viruses: virology study of parasites: parasitology study of fungi: mycology NOT ALL BACTERIA ARE PATHOGENS (disease causing) antibiotic: used to treat bacterial infections and diseases (penicillin‚ mold‚ 1929) bioremediation: using bacteria to clean up toxins pathogenesis: ability of an organism to be pathogenic virulence factors: toxins‚ receptors‚ cellular composition innate immune response v.s
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harvard.edu/node/222 BSP‚ S. (2010). How is EM different from light microscopy? Retrieved April 25‚ 2015‚ from http://bsp.med.harvard.edu/node/222 Tortora‚ G.‚ Funke‚ B.‚ & Case‚ C. (2010). Staining Techniques. Retrieved April 25‚ 2015‚ from http://www.cliffsnotes.com/sciences/biology/microbiology/microscopy/staining-techniques
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preparation has been widely used however‚ the safety of its oral use against normal microflora‚ is still ambiguous despite traditional practice hence‚ this study. The disc diffusion method was used to determine the antibacterial activity against gram positive and gram negative bacteria (standard strains and clinical isolates). Antibacterial activity was tested on E. coli‚ S. aureus‚ B. subtilis and P. aeruginosa. Results show that there was no formation of any zone of inhibition as observed after 24 hrs
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Activities/Tasks/Responsibilities | Problem(s) Encountered | Action(s) Taken | Personal Reflection | BacteriologyJuly 4- July 31 | Receiving of specimen‚ Logging‚ Processing of specimen‚ Inoculation into media‚ biochemical testing‚ identification of organism‚ gram staining‚ AFB staining‚ releasing of results‚ culture sensitivity testing. | No major problems were encountered during my rotation for Bacteriology. Minor problems like unfamiliarization of SOP’s and other procedures are encountered. | Asking proper procedures
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