inoculating loop or needle‚ Bunsen burner‚ glass slides‚ lens paper‚ bibulous paper‚ regular tap water‚ along with reagents Crystal violet‚ Gram’s Iodine‚ 95% ethyl alcohol‚ and safranin. The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this‚ I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable
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results‚ the isolated bacteria were tentatively identified as Agrobacterium strains. B.Characterization of Agrobacterium tumefaciens: Biochemical tests: biochemical features of the selected isolates were given in the table 1. The Gram reaction indicates selected isolated are Gram negative. All the six isolated are positive
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ATM (STP). A Gram stain was then carried out to differentiate the unknown sample from a broad class to a more specific category of bacteria. The Gram stain distinguishes between Gram-positive and Gram-negative bacteria based on the composition of the cell walls. Gram-positive bacteria appear purple and Gramnegative bacteria appear pink after staining. The first Gram stain produced unsatisfactory results and was then repeated with a clear indication of negativity. Light pink staining was evident
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3.3 Microbiological Analysis 3.3.1 Bacteria count using Heamocytometer This method was used to determine the of three types of dessert but does not differentiate the type of bacteria. 25 g of food samples will be homoginesed in a sterile stomacher bag and shaken for two minutes with 225 ml of peptone water to obtain the food mixture. Using separate sterile pipets‚ decimal dilutions of 10-2‚ 10-3‚ 10-4‚ 10-5 will be prepared and others as appropriate‚ of food homogenate by transferring 10 ml of previous
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Kobayashi et al.‚ 1997‚ also have reported the use of Bacillus chitinase for the production of ulcerative colitis and other
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CHAPTER ONE INTRODUCTION AND LITERATURE REVIEW 1.0 Ginger Ginger is a knotted‚ thick‚ beige underground stem (rhizome). The stem extends roughly 12 inches above ground with long‚ narrow‚ ribbed‚ green leaves‚ and white or yellowish-green flowers. The important active components of the ginger root are thought to be volatile oils and pungent phenol compounds (such as gingerols and shogaols). 1.1 Parasitological examination of stool specimen This is the examination of intestinal parasites
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Our main goal was to test organisms’ resistance and susceptibility to bacteria‚ and whether the cell wall of bacteria exposed the membrane to pathogen or protect it from pathogens. I thought P. aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used
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and was moved to the incubator for 48 hours at 35 degrees Celsius. The same procedure was used on all of the samples. Following the 48 hour incubation period‚ the Petri plates were observed for colony morphology‚ and the results were recorded. A Gram
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to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed
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Chapter 1. Microbiology – Diversity of Organisms Microorganisms- too small to be seen with the unaided eye “germ”- rapidly growing cell Microbes in our lives Pathogenic- disease causing Decompose organic waste Producers in ecosystem (photosynthesis) Produce industrial chemicals such as ethanol and acetone Produce fermented foods ( vinegar‚ cheese‚ bread) Produce products used in manufacturing (cellulose) and treatment (insulin) Designer Jeans: Made by Microbes? Stone washing- Tricoderma
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