Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Hypothesis: I believe that the room temperature activity will produce the most activity. Materials: 3% hydrogen peroxide‚ a household blender‚ potatoes (3 medium size potatoes‚ that are peeled and quartered)‚ tap water‚ 8 test tubes‚ 8 transfer pipets‚ sugar packets‚ salt‚ a small saucepan‚ a refrigerator with a freezer‚ stove‚ 4 clear plastic cups‚ tongs (to remove
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Investigating an enzyme controlled reaction: catalase and hydrogen peroxide concentration Objectives * to investigate the effect of substrate concentration on the activity of catalase * to evaluate a practical protocol Procedure SAFETY: Wear eye protection and protect clothing from hydrogen peroxide. Rinse splashes of peroxide and pureed potato off the skin as quickly as possible... Investigation * Use the large syringe to measure 20 cm3 pureed potato into the conical flask.
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Aim: To find the effect of temperature on enzymes‚ using a potato as a catalyst. The source of catalase is in the potato cells. 2H2O2 → O2 + H2O Planning: Introduction: An Enzyme is any one of many specialised organic substances‚ composed of polymers of amino acids‚ that act as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organisms Enzymes are classified into several broad categories‚ such as hydrolytic‚ oxidising‚ and reducing‚ depending
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conditions affect the rate at which enzymes accelerate/cause reactions In this lab students measured the height of the foam after catalysis between catalase (enzyme) and 7 other (solutions) to determine which solution had the fastest reaction rate.. The control variable of the experiment would be the solution of only hydrogen peroxide‚ water‚ and catalase. The independent variables of the experiment were the abiotic factors such as PH level‚ temperature‚ and the amount of salt within the environment
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Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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concentration of the catalase which the disk is soaked in‚ it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas. Prediction: Since the rate of reaction can be lowered by the addition of catalysis such as an enzyme. Moreover to increase the effectiveness of an enzyme one must add additional amount of enzyme due to saturation. Therefore the 100% will have the greatest effect because is has the highest concentration of the catalase.
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its ability to function. The purpose of this experiment was to observe how changes in these external environment factors affect an enzyme’s reaction rate. Three different levels of each variable were used to see how liver enzyme catalase reacts with the substrate hydrogen peroxide. This experiment allows scientists to compare low‚ intermediate‚ and high levels of temperature‚ pH‚ and ionic concentration to determine which level provides an optimal condition versus which level causes the enzyme to
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Investigating How Different Concentration Effects The Rate Of Reaction. Strategy A Possible Factors * Source of catalase * Concentration * Surface Area of enzyme * Concentration of enzyme * pH * Temperature Chosen Factor We chose to investigate the concentration of enzyme as we had previously investigated the optimum temperature for catalase in the preliminary investigation. Concentration of enzyme is also fairly easy to investigate‚ as you need to only increase
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classified into several categories such as hydrolytic‚ oxidizing‚ and reducing. One of the simplest biological reactions by an enzyme is the breaking down of hydrogen peroxide by the enzyme catalase. This reaction is a very essential life process since the metabolic waste product‚ hydrogen peroxide‚ is toxic to the cell in high amounts. Catalase is a very efficient enzyme‚ it has one of the highest rates of reaction among all of the enzymes. Enzymes have high specificity‚ generally enzymes catalyse
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animals‚ by inhibiting the activities of antioxidant enzymes like catalase‚ superoxide dismutase‚ glutathione peroxidase and glutathione reductase. However‚ it is not understood how BPA and NP interact with these enzymes and inhibit their functions. Hence‚ it would be significant to check‚ whether binding sites are available for NP and BPA in antioxidant enzymes. In the present study three-dimensional structures of antioxidant enzymes‚ catalase‚ superoxide dismutase‚ glutathione peroxidase and glutathione
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