FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment‚ write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a household blender •potatoes (3 medium size potatoes‚ that are peeled and quartered) •tap water •8 test tubes •8 transfer pipets •sugar packets •salt •a small saucepan •a refrigerator with a freezer •stove •4 clear plastic cups
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concentration‚ temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction. Materials and Methods: Experiment 1‚ 2‚ & 3 Experiment 1 examined the effects of concentration on catalase activity. Experiment 2 examined the effects of concentration in temperature on catalase activity Experiment 3 examined the effects of pH on catalase activity The eudiometer was filled with water and inserted
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In this experiment the substrate is going to be hydrogen peroxide(H2O2) and the enzyme is going to be catalase. Hydrogen peroxide(H2O2) is a product of aerobic cellular respiration and is considered a free radical. This means that too much of hydrogen peroxide can affect the organelles. In order to stop damages caused by hydrogen peroxide‚ the enzyme catalase changes hydrogen peroxide(H2O2) to water(H2O) and oxygen gas(O2). Catalase is present most animal cells. The reaction is ……………….
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of an enzyme(catalase). Materials Potato‚ mortar & pestle‚ 4 test tubes‚ test tube rack‚ spatula‚ hydrogen peroxide 6%‚ 3%‚ 1.5%‚ pipettes‚ scalpel‚ ruler. Procedure 1. Cut using scalpel several raw potato cubes each measuring 1 cubic centimeter (cc). 2. Each cube was ground with a mortar & pestle and then added to a test tube using a spatula. 3. Test tube 1 was left as the control with no added hydrogen peroxide. 4. Different concentrations of hydrogen peroxide solution were
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or active oxygen species are oxygen that is more energetic compared to the normal molecular oxygen. They are unstable and are formed either through loss of electrons or addition of protons. For example super peroxide anion (O2.-) and protonated per hydroxyl radical (H2 O2-)‚ hydrogen peroxide‚ H2 O2 and hydroxyl radical OH. (Smirnoff‚ 2007). . When these species are in the body‚ they become threats to cells
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enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase After the lab‚ one should be able to: • Measure the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on reaction rates of an enzyme-catalyzed reaction in a controlled experiment; and • Explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: When one mixes catalase and hydrogen peroxide with sulfuric acid for a short period of time‚
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individual type of enzyme does a specific job‚ and they do not die‚ they are reused. For example‚ catalase is an enzyme found in almost all living cells that will break down hydrogen peroxide and turn it into water and oxygen. The breakdown of Hydrogen peroxide happens when it gets released from metabolism‚ and Materials: Yeast Solution 4 Test tubes Graduated cylinder 1 Bottle of Hydrogen peroxide Hydrochloric acid Hot water bath Ice Bath Test tube rack Test tube holder Safety: Wear goggles
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An enzyme that catalyzes the reduction of hydrogen peroxide is specifically known as catalase. This chemical reaction that is most catalyzed by catalase H2O2 decomposition to form water (H2O) and oxygen (O2). This chemical reaction is represented by the formula: 2 H2O2 → 2 H2O + O2 (gas) Hypothesis: If the chemical reaction‚ H2O2 decomposition
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enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure: 1. Place all 5 glass tubes face up in the drying rack 2. Use tweezers and soak one potato cube into water
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11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity studies reflect
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