Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
Premium Catalase Enzyme Chemical reaction
maintain a stable internal environment (homeostasis) for optimal cell and tissue metabolism. They do this by separating urea‚ mineral salts‚ toxins‚ and other waste products from the blood. They also do the job of conserving water‚ salts‚ and electrolytes. At least one kidney must function properly for life to be maintained. Six important roles of the kidneys are regulation of plasma ionic composition‚ regulation of plasma osmolarity‚ regulation of plasma volume‚ regulation of plasma hydrogen ion concentration
Premium Kidney
to water of the cell membrane and the capillary wall? Both the plasma membrane and the capillary wall are permeable to water. 13. Drinking a liter of water will cause the osmolarity of blood plasma to: decreases 14. If someone runs a marathon through a desert while eating pretzels and not drinking water‚ their plasma osmolarity: rises and becomes hypertonic to the interstitial and intracellular fluid compartments. 15. As an individual becomes dehydrated‚ the net movement of fluid in their body is
Premium Blood Carbon dioxide Potassium
:12 November 2014 INSTRUCTOR: Miss Ellia Kartini Mujar OBJECTIVES 1. To demonstrate reduction-oxidation reaction on living tissue. 2. To compare enzyme catalase on plant and animal tissues. 3. To measure the rate on enzyme reaction. 4. To measure enzyme reaction. INTRODUCTION In this lab activity‚ we explore on the enzyme that is found in the cells of many living tissues. The enzyme is catalase. Catalase functions in speeding up reaction in our cells that break down hydrogen peroxide‚ a toxic substance
Free Enzyme Hydrogen peroxide Catalase
equal concentration of water molecules. MATERIALS: 1. Potato slices 2. Elodea leaf/ onion scales 3. Razor blades 4. Digital balance /string /ruler 5. Varying molar of sucrose solutions (0.01 M‚ 0.02 M‚ 0.05 M‚ 0.1 M‚ 0.3 M‚ 0.5 M) 6. Tissue papers 7. Test tubes 8. Watch glass 9. Forceps PROCEDURE: Task 1: The effect of osmosis in potato cells 1. The potato was cut in to 3 slices for each solution and placed in 7 Petri-
Premium Osmosis
tube‚ and placed on ice. It was then taken to a lab and spun for 3 minutes on a microcentrifuge. The serum from the microcentrifuged hemolymph was taken back to the General Physiology Lab in Duffy and placed into osmometer‚ which measures the osmolarity. This is done by freezing the sample of hemolymph and the time that it takes to defrost depicts the amount of osmoles. Water analyses for the three tanks determined the temperature ºC‚ pH‚ salinity (ppt)‚ oxygen levels and the ammonia content
Premium Hydrogen Lobster Ammonia
Practical Work Nº2: “Different methods to control osmosis” Aim: observe and test the process of osmosis through different kinds of methods: the weigh (potato)‚ the density (beetroot) and under the light microscope (onion). Hypothesis: according to the encyclopedia definition osmosis is the diffusion of a liquid (most often assumed to be water‚ but it can be any liquid solvent) through a partially-permeable membrane from a region of high solvent potential to a region of low solvent potential. Thus
Premium Concentration Osmosis Laboratory glassware
Urinary System: Anatomy Review 1. Name the organs in the urinary system: 1. 2 kidneys 2. 2 ureters 3. bladder 4. uethra 2. The kidneys are retroperitoneal (behind the peritoneum)‚ lying against the dorsal body wall in the upper abdomen. 3. The adrenal gland sits atop the kidneys. Blood vessels enter and leave the kidney at the renal cortex. 4. The functional units of the kidney are the nephrons. They are called renal pyramids if they are located mainly in the cortex. They are called renal
Premium Nephron Kidney
The effect of Sodium Chloride (NaCl) salt concentration on osmosis in potato cells The movement of substances in plant cells involves many processes and systems‚ all of which may affect the plant bio-chemically and physically‚ and one of these processes is osmosis. Osmosis is the flow of water through a semi-permeable membrane of a cell moving from an area of higher water potential to an area of lower water potential until reaching equilibrium known as isotonic. Before reaching the point of being
Premium Sodium chloride Chemistry Chlorine
observe plant cells as to their shapes‚ structures and function. Materials: Microscope cover slip glass slide water onion Iodine soln. hydrilla salt tomato razor blade Medicinal dropper epidermal tissue of rhoeo-discolor Procedure: 1. Onion Cell 1. Prepare an onion bulb 2. Place a drop of water at the center of the slide. 3. Pell off a thin layer from the inner surface of onion bulb [Allium cepa]. 4. Put a cover slip on the
Premium Cell wall Cell Water