Murphy Nmezi August 9‚ 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose
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NaOH to our buffer 1 solution. The original pH was 5.32‚ with 10mL of buffer and 10mL of DI water. We added 5mL of NaOH and the pH changed to 12.12. If 5 mL changed the pH by about 7‚ then we predict that about 2 mL of NaOH would change the pH by about 2. This prediction is based on the fact that 2.5mL is half of 5mL‚ and 3.5 is half of 7‚ so adding a little less than 2.5mL would give us a difference of about 2. For the buffer 2 solution‚ the original pH was 5.82 with 10mL of buffer and 10mL of DI
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of Temperature and pH on Catalase Activity INTRODUCTION Enzymes are organic catalysts that spur metabolic reactions. The presence of an enzyme within a cell is essential in order for any sort of reaction to take place. All enzymes are complex proteins that act in an organism’s closely controlled internal environment. In such a homeostatic environment‚ the temperature and the pH (concentration of hydrogen ions)‚ remain within a fairly narrow range. Extreme variations in pH and temperature denature
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the soil needs to have a relatively high pH‚ high organic matter content‚ and moist conditions. Specific soil orders such as Vertosols‚ Kandosols‚ Sodosols‚ Rudosols‚ and Calcarosols‚ lack the requirements for phosphorus nutrition. The availability of phosphorus heavily relies on soil pH; the pH range for highest availability is between 5.5 and 7.0‚ whereas‚ Queensland and South Australia are both out of this range. Queensland has acidic soils‚ where the pH is typically between 4.0 and 5.5‚ and South
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EFFECT OF pH ON ENZYMATIC ACTIVITY Jenelle C. Faustino‚ John Gambit B. Garcia‚ Fatima S. Jusay‚ Oliver Alexander B. Lao and Eunice L. Licudine Group 4 2 E Medical Technology Biochemistry Laboratory ABSTRACT Enzymes are substances that are produced by living organisms and act as catalysts in order to speed up or chance a chemical reaction without changing itself at the end of the reaction. Invertase was extracted first from baker’s yeast. Determination of the effects of changes in pH on enzymatic
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of the chemical composition of cells is the pH of their environment. Most cells operate in a narrow pH range. That is‚ pH values above or below a certain value may cause a cell distress or may even be fatal. To help maintain relatively constant pH levels‚ living systems use buffers. A buffer is a combination of a weak acid and a weak base that function together to minimize changes in the pH of a solution. In today’s lab‚ we will learn how to measure pH and demonstrate how buffers work. II) Procedure
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The purpose of this experiment was to test how effective certain homogenates were as buffers. Buffers are devices that keep pH within maintainable boundaries so something can function. When something is too basic (has too much OH-) the buffer adds H+ and vice versa in order to create water to keep the pH at an acceptable range. Each group (I was with William Yung for this experiment) was tasked with testing one homogenate. The homogenate tested by our group was liquid spinach. Each team added HCl
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Investigation of pH titration curves (Topic 8) Criteria Assessed: DCP‚ CE Time allowed: 1.5h Name: ________________________ Internal Assessment Criteria Aspect 1 Aspect 2 Aspect 3 Total DCP CE Aim: The aim of this practical is to plot and investigate the pH titration curves for the titration of a strong acid with a strong base and of a weak acid with a strong base‚ and find Ka of the weak acid. Procedure: Part I 1. Pour 30 ml of the HCl solution of unknown concentration in a 100 ml beaker
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conjugate acid that will have a minimal change in pH when a strong base or acid is added to it. The results and observations of this experiment proved (a) the occurrence of the common ion effect through the determination of pH and titration and (b) how minimal the change in the pH of a buffer solution is upon the addition of a strong base and a strong acid. II. KEYWORDS: Common Ion Effect‚ Buffering Effect‚ Buffer Solutions‚ Ionic Equilibrium‚ Solubility‚ pH III. INTRODUCTION The process in which an
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Calculation for prepare 0.1 M potassium phosphate buffer at pH 6 3.4007g of potassium phosphate was weighed and placed in 300 mL beaker. Then‚ 125 mL of water was added into the beaker that contained potassium phosphate. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution‚ and the data was documented at pH 4.6. This was the starting point. Next‚ 1M NaOH was
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