a scarce amount of glucose (100 mg). Lactase enzyme reaction when exposed to different pH levels The purpose of this experiment is to analyze how different pH levels affect lactase activity. The three different pH levels that we will test are: pH 4‚ pH 6‚ and pH 10. To determine if lactase was able to function and produce glucose‚ we will use glucose test strips. Table 3. Lactase functioning affected by pH
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Murphy Nmezi August 9‚ 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose
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temperature‚ the pH‚ the turbidity‚ the conductivity‚ and the dissolved oxygen (A.K.A. DO) (1). Those are the focus of this study. Temperature and dissolved oxygen are both related to each other. The dissolved oxygen is inversely proportional to the temperature of the water. Anything decaying in the water will decrease the dissolved oxygen. A value less than 5 mg/L of dissolved oxygen is hard on the fish. When the dissolved oxygen has a value less than 2 mg/L‚ the fish will die (1). pH helps not
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500 482. Ph : 040-2331 1224‚ 233 10539‚ www.lumbiniconstructions.com --------------------------------------- Janpriya Engineers Syndicate Ltd. Janapriya House‚ 3-6-115 Himayat Nagar‚ Hyderabad 500029 Andhra Pradesh‚ INDIA Ph : +91-40-23222999‚ 23222666‚ 23222333. www.janapriya.com --------------------------------------- Ashoka Builders # 8-2-120/112/P/7‚ 3rd Floor Park View Estate‚ Opp KBR Park Above Talwar Hyundai Road No:2‚ Banjara Hills‚ Hyderabad - 500 034. Ph :23607272
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rain is measured using a scale called “pH.” The lower a substance’s pH‚ the more acidic it is. What is ph? A figure expressing the acidity or alkalinity of a solution on a logarithmic scale on which 7 is neutral‚ lower values are more acid‚ and higher values more alkaline. The pH is equal to log10 c‚ where c is the hydrogen ion concentration in moles per lite Describe the ph range and list various things that correspond to the various ph levels. The pH scale goes from 0 - 14‚ with 7 being neutral;
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NaOH to our buffer 1 solution. The original pH was 5.32‚ with 10mL of buffer and 10mL of DI water. We added 5mL of NaOH and the pH changed to 12.12. If 5 mL changed the pH by about 7‚ then we predict that about 2 mL of NaOH would change the pH by about 2. This prediction is based on the fact that 2.5mL is half of 5mL‚ and 3.5 is half of 7‚ so adding a little less than 2.5mL would give us a difference of about 2. For the buffer 2 solution‚ the original pH was 5.82 with 10mL of buffer and 10mL of DI
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the soil needs to have a relatively high pH‚ high organic matter content‚ and moist conditions. Specific soil orders such as Vertosols‚ Kandosols‚ Sodosols‚ Rudosols‚ and Calcarosols‚ lack the requirements for phosphorus nutrition. The availability of phosphorus heavily relies on soil pH; the pH range for highest availability is between 5.5 and 7.0‚ whereas‚ Queensland and South Australia are both out of this range. Queensland has acidic soils‚ where the pH is typically between 4.0 and 5.5‚ and South
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The purpose of this experiment was to test how effective certain homogenates were as buffers. Buffers are devices that keep pH within maintainable boundaries so something can function. When something is too basic (has too much OH-) the buffer adds H+ and vice versa in order to create water to keep the pH at an acceptable range. Each group (I was with William Yung for this experiment) was tasked with testing one homogenate. The homogenate tested by our group was liquid spinach. Each team added HCl
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of the chemical composition of cells is the pH of their environment. Most cells operate in a narrow pH range. That is‚ pH values above or below a certain value may cause a cell distress or may even be fatal. To help maintain relatively constant pH levels‚ living systems use buffers. A buffer is a combination of a weak acid and a weak base that function together to minimize changes in the pH of a solution. In today’s lab‚ we will learn how to measure pH and demonstrate how buffers work. II) Procedure
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of Temperature and pH on Catalase Activity INTRODUCTION Enzymes are organic catalysts that spur metabolic reactions. The presence of an enzyme within a cell is essential in order for any sort of reaction to take place. All enzymes are complex proteins that act in an organism’s closely controlled internal environment. In such a homeostatic environment‚ the temperature and the pH (concentration of hydrogen ions)‚ remain within a fairly narrow range. Extreme variations in pH and temperature denature
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