enzyme‚ just like any other enzyme‚ has an optimum PH and temperature range in which it is most active‚ and in which the substrate binds most easily. The purpose of this experiment was to determine (1) the reaction rate of an amylase enzyme in starch and (2) the environmental factors that can affect the enzymatic activity. The hypothesis‚ in relation to the enzymatic activity by variables such as the substrate concentrations‚ temperature‚ PH and chemical interactions on the rate of reaction
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Part 1 Part 1 of the experiment investigated the effect of different temperatures on beetroot cell membranes (a type of plant cell). Through this experiment‚ the process of diffusion and osmosis was in action. Various temperatures ranging from low temperatures to high temperatures such as -5⁰C‚ 5⁰C‚ 30⁰C‚ 50⁰C and 80⁰C were used to investigate the temperature effects on beetroot cell membranes. The hypothesis predicted that the higher the temperature the darker the beetroot substance and the lower
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SCI103 Phase 1 Lab Report Title: Measuring pH Levels Instructions: Enter the Virtual Lab‚ and conduct the experiments provided before going out into the filed for additional research. Please type your answers. When your lab report is complete‚ submit it to the Submitted Assignments area of the Virtual Classroom. Part I: Answer the following questions while in the Phase 1 lab environment. Section 1: You will be testing 4 known solutions for pH levels using a standard wide-range indicator
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The tubes were filled with 3 ml of their corresponding pH phosphate buffer. 10 drops of catechol and 10 drops of potato juice‚ which contains catecholase‚ were added to each of the seven tubes. The tubes were covered with Parafilm‚ stood for 5 minutes‚ and mixed every minute. After the 5 minutes‚ data was recorded based on the intensity of color with the “0‚+‚++‚+++”
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Hot Plate 10ml of 0.1M HCl 10ml of 0.01 M HCl 10ml of 0.001 M HCl 10ml of 0.001 M NaCl 10ml 0f 0.01 M NaCl 10ml of 0.1 M NaCl 10ml of tap water 9 Test Tubes (The Effect of pH) Graduated Cylinder 6 Discs of Frozen Beetroots (3 per experiment) 10 ml of Acetone per experiment Procedure: The Effect of pH 1. Top and bottom of beetroot were cut off by scalpel. The cork borer was used to cut out about 3-4 cores‚ then each core was cut into uniform discs about 2mm thick. 2. Discs were
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Title : Measuremnt of pH With Indicators Aim : 1.To investigate concentration of hydrogen ions in hydrochloric acic and colour changes of indicators 2.To determine standard solutions and the unknowns Variables Independent variable : Concentration of hydrogen ions in hydrochloric acid.The presence of hydrogen ions is varied by using 5 different concentration of hydrochloric acid of 0.1mol/L‚0.01mol/L‚0.001mol/L‚0.0001mol/L‚0.0000mol/L Dependent variable : Colour changes of indicators
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a narrow range that is slightly alkaline. The normal pH of arterial blood is 7.35 and 7.45. Acids are continually produced during metabolism. Several body systems including buffers‚ the respiratory system and the renal system are actively involved in maintaining the narrow pH range necessary for optimal function. Buffers help maintain acid bases balance by neutralizing excess acids and bases. The lungs and the kidneys help maintain a normal pH by either excreting or retaining acids or bases. (1)
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2014 Zamir Deen Virtual Chem Lab 6-2 & 6-3 Ranking Salt Solutions by pH 6-2: Ranking Salt Solutions by pH In this assignment you will be asked to rank aqueous solutions of acids‚ bases‚ and salts in order of increasing pH. This is most easily done by first identifying the strong acids that have the lowest pH‚ the strong bases that have the highest pH‚ and the neutral solutions that have a pH near 7. The weak acids will have a pH between 1 and 6 and the weak bases between 8 and 14. The exact order
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Biology 181 Ana Marti-Subirana Identification of unknown a-Amylase through testing different temperatures and pH values to detect the absorbance of maltose. Introduction: Enzymes are biological catalysts‚ mainly proteins for this experiment‚ generated by an organism to speed up chemical reactions. They have active sites on which the substrate is attached‚ and then broken up or joined. For this experiment we are going to work with the enzyme a-amylase. Amylase is an enzyme that breaks
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separate container. The pH of acid rain around 4.0. Acid rain is caused by a chemical reaction when sulfur dioxide and nitrogen oxide are released into the air. When these compounds are mixed with rain it is known as acid rain. Acid rain mostly affects the ocean and animals within that area.
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