Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
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optimal ranges under which the enzyme activity ismaximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme willincrease until it reaches optimum pH range (pH 7) because the enzyme is less denaturedwhen it reaches the preferred pH level‚ and after this it will decrease because the activesite will change in shape and it will no longer accept substrates.Temperature factor prediction:
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molecules Donor donates covalently bonded hydrogen to an electronegative acceptor atom 6. What does the pH of a solution refer to? The amount of Hydrogen ions found in a solution. 7. Which pH values are considered acidic‚ basic and neutral? 1-6 acidic 7 neutral 8-14 basic 8. What does the pKa of an acid or base refer to? Strength of acid 9. What is a buffer and at what pH value is a buffer most effective?
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Diazonium ions should be used immediately. Stored compounds can decompose‚ sometimes violently. DO synthesis inside the fumehood. MATERIALS Sodium nitrite Conc‚ sulphuric acid aniline N‚N-dimethylaniline nitroaniline phenol naphthol pyridine pH paper beaker‚ test tubes graduated cylinder filter paper PROCEDURES A. Preparation of Diazonium Salt Since these are immediately reacted‚ they should be prepared in parallel fashion‚ i.e. one vessel per prospective reaction. Into each vessel
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temperature affect enzyme activity? I think that at normal body temperature‚ regular enzyme activity will occur. 5] What about pH levels? I think that at a balanced pH level (pH 7) like water‚ enzyme activity will be at the highest. Objectives: -To demonstrate the activity of an enzyme in living tissues -To learn how changes in temperature and pH affect the activity of a catalase -To experimentally test for the presence of catalase in living tissue -To analyze factors
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Salts 1. I am pure water. When heated my pH (increases‚ decreases)‚ because more of my water molecules dissociate. 2. I am a 0.020 M solution of weak acid‚ HA. If I only dissociate to the extent of 1.50%‚ what is the value of my Ka? 3. I am a 0.20 M solution of hydrocyanic acid‚ HCN‚ with a Ka of 4.93 x 10¯ 10 . What is my pH? 4. I am a buffer made from 0.10 M acetic acid and 0.15 M sodium acetate. If the Ka for acetic acid is 1.77 x 10¯ 5 ‚ what is my pH? 5. I am a substance who turns blue litmus
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enzyme concentration‚ temperature and pH on hydrolysis and solubilization of corn gluten as well as enzyme stability. The corn gluten was hydrolyzed by Alcalase enzyme (a bacterial protease produced by a selected strain of Bacillus licheniformis) that was chosen among five commercial enzymes examined. The optimum process conditions for hydrolysis and solubilization were obtained as 30 g L–1 substrate mass concentration‚ 2.5 mL L–1 enzyme concentration‚ 55 °C and pH 8. Under these conditions‚ the values
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your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation
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single sensor channel‚ and were difficult to assemble due to the use of discrete components. The measurement parameters consisted of temperature‚ pH or pressure‚ and the first attempts of conducting real-time noninvasive physiological measurements suffered from poor reliability‚ low sensitivity‚ and short lifetimes of the devices. The first successful pH gut profiles were achieved in 1972‚ with subsequent improvements in sensitivity and lifetime. Single-channel radio telemetry capsules have since
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Determining the equilibrium constant from pH values of changing concentrations of ethanoic acid Design Research question: How will altering the concentrations of ethanoic acid affect the pH value‚ and‚ in-turn‚ the equilibrium constant? Background information: When weak acids react‚ the reaction typically does not go to completion. Rather‚ the system goes to an intermediate state in which the rates of the forward and reverse reactions are equal. Such a system is said to be in chemical equilibrium
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