of LDH using gel filtration chromatography with Sephacryl S-200 HR. 2. To determine the activity and the absorbance of LDH in gel filtration chromatography. 3. To determine the molecular weight of the LDH. MATERIALS AND METHODS Please refer to page 45 to 46 of BCH204/304/3204 May 2013 session laboratory manual. RESULTS Volume assayed (µL) 10 Dilution used (if any) 10x ∆ Absorbance/ ∆ min 0.0221 U/mL 10.66 Total mL loaded on column 5 mL Total units loaded on column 53.30 Table1
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finding its absorbance through the use of spectrophotometer. Four known concentrations were prepared; 2.5 x 〖10〗^(-3) M‚ 6.25 x 〖10〗^(-4) M‚ 1.25 x 〖10〗^(-4) M‚ 6.25 x 〖10〗^(-4) M. The solutions were placed on the spectrophotometer to determine absorbance together with the unknown. Distilled water was placed before each trial to ensure the accuracy of results. Determining the concentration of the unknown sample was done by drawing a graph of the concentration against the absorbance and was found
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Fresh potatoes extract in phosphate buffer pH6 containing the enzyme: polyphenol-oxidase‚ was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan‚ R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts
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The main technique used in this experiment is Spectroscopy. The three equations used in this lab are: Y = mx + b‚ where y = absorbance‚ x = concentration‚ and b = 0 using a calibration curve to establish the range of concentration values over which Beer’s law is valid A=Ɛbc‚ where A = absorbance‚ Ɛ = molar absorptivity‚ b = path length‚ and c = concentration Absorbance is defined as a measure of the capacity of a substance to absorb light of a specified wavelength. Concentration is defined as
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Chemistry of Natural Waters Lab Report Lauren Braas Thursday November 12‚ 2009 Chemistry 111 Group members: Sheila Azutillo Markeisha Brown TA: Darlene Biziak Introduction Water is such an important substance needed by all living species for survival. Freshwater is needed for agriculture‚ hydroelectric power‚ and drinking water. The United States uses 9 X 1011 liters of freshwater everyday.1 Freshwater is not completely pure. Its composition is based on dissolved solutes ranging
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will begin taking readings of %T. Leave the sample tube in the instrument throughout the data collection process. Obtain readings of %T every minute for at least 15 minutes. Record %T values to one decimal place‚ as this allows you to convert to absorbance and retain three significant figures. When the 15th and final reading has been taken‚ remove the sample from the instrument and prepare to re-zero the instrument with a new NaOH blank in another test tube. Then repeat the entire 15-minute data
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Introduction The population of a bacterium will grow successfully in a particular pattern known as ‘logarithmic growth’ if the location is appropriate and the proper nutrients are present. The bacteria numbers will increase and most likely continue to double in this area over time with optimal conditions. Optimal conditions meaning that there are no dangers or limiting factors‚ to inhibit growth in this area‚ such as disease‚ predators‚ competition or lack of food. At first the bacteria should
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Proteins Biuret Test Results. Figure 1 is showing both graphs of results from the spectrophotometer. Figure 1A is the results without the unknown plotted. By using the best fit line and equation the unknown substance was found to have .75 absorbance and 11.5 mg/mL protein concentration. Figure 1B shows the newly found data of the unknown plotted with X.
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levels‚ and by a competitive inhibitor (hydroxylamine). The enzyme activity was measured in various ways depending on the activity. Temperature effects showed the amount of activity and determined the optimal pH level. The spectrometer showed the absorbance units at 500nm and determined the optimal temperature. The temperatures tested where 4ºC (approximately refrigerator temperature)‚
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using colorimetric analysis Materials On the sheet attached Procedure On the sheet attached Results Standard (mg/L) | Absorbance | 10.0 | 0.354 | 7.5 | 0.308 | 5.0 | 0.230 | 2.5 | 0.193 | 0.0 | 0 | unknown | 0.161 | Calculations 1. What is the concentration of phosphorus ions‚ in mg/L‚ in the initial 250 mL of fertiliser solution? y = 0.0399x Absorbance of unknown = 0.161 0.161 = 0.0399x x = 4.035 4.035 x 10 = 40.35 = 40.4 mg/L 2. Use
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