on the Genesys spectrophotometer . We then measured the 0.500 M Cu2+ absorbance of wave length every 25 nm until reaching 950 nm. We then found our wavelength at which A is a maximum and repeated measuring every 5 nm. We then diluted our solution further using a pipette adding 20 ml‚ 10 ml‚ and 5 ml to three separate 25 ml volumetric flask and diluted to the calibration line and swirled vigorously. We then measured the absorbance of wavelength of each vial. We then weighed out 2.0255 g of the unknown
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The effect of differing wavelengths of visible light on the photosynthetic activity of Beta vulgaris Introduction Photosynthesis is a crucial biological process that occurs within the chloroplasts of plant cells where CO2 + H2O + Sunlight C6H12O6 + O2. The chloroplasts use light‚ an electromagnetic energy source‚ to produce food for the plant in the form of sugar molecules. During photosynthesis‚ the excited electrons from the light pass through proteins in the electron transport chain (ETC)‚ where
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Abstract The reaction rate of an enzyme can be affected by many factors‚ and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature constant. Our findings illustrate that the rate of enzyme activity is only influenced by
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BIO-301 04-08-2013 Introduction and Background: Photosynthesis is the process in which light energy is converted to chemical energy‚ and allows plants to grow‚ flower and produce seed. The process of photosynthesis requires an organelle called chloroplast and a pigment called chlorophyll The energy of the light is absorbed by chlorophyll and in turn supplies the plant with energy to transform carbon dioxide and water into oxygen and carbohydrates. (lab handout)
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Bacterial Energetics and Membranes Abstract The Mg2+/Ca2+ ATP synthase present in all bacterial membranes‚ particularly E. coli‚ couples ATP synthesis to the proton (H+) gradient produced by the ETC‚ a process known as oxidative phosphorylation. The gradient acts to power the ATPase‚ so that it may phosphorylate ADP to produce ATP. The reverse reaction of this process‚ or hydrolysis of ATP into ADP and Pi‚ may be used to observe ATPase activity when the resulting Pi is quantitatively measured
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First‚ the buffer was prepared by using the formula as follows: Figure 1: Calculation for prepare 0.1 M potassium phosphate buffer at pH 6 3.4007g of potassium phosphate was weighed and placed in 300 mL beaker. Then‚ 125 mL of water was added into the beaker that contained potassium phosphate. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution
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Hydrogen peroxide and iodide ions Aim: To determine the rate equation for the reaction between Hydrogen peroxide and iodide ions‚ and to investigate the effects of a catalyst and temperatures on the reaction and to derive the activation enthalpy. Background knowledge: 1Hydrogen peroxide reacts with iodide ions producing iodine‚ when in an aqueous acid solution. H2O2 (aq) + 2I- (aq) + 2H3O+(aq) I2 (aq) + 2H2O(l) To detect iodine you can look at the color shown by the addition of starch
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Experiment 1 Lab Report Title: Determination of caffeine content in tea bag sample by UV spectroscopic method Higher diploma in Pharmaceutical Science-Year 1 AS114202-1TM Fundamental Analytical Chemistry ASD3008 Lab Group 6: Ng Chung Hin 130439161 Wong Tin Yau Tony 130252529 Ng Wai Kin 130031247 Tam Chun Kit 130191034 Mau Kwan Yiu 130395762 Date of Experiment: 7/3/2014 Content Introduction Objectives Methodology Results Discussion Conclusions References Introduction
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DCPIP in each. • Making blank‚ pipette 1mL chloroplast and add ascorbic acid • Set spectrophotometer to 595nm • Pipette 1mL chloroplast into each cuvette‚ make sure light is maximized • Remove foil‚ determine absorbance at time zero. Place in front of light at lower intensity. • Take absorbance for same tube at 2min intervals for 8min or until 0.5 ABS • Repeat at each light intensity‚ 100. Expose each cuvette for 4 min or until 0.5ABS • Using molar extinction coefficient 0.014‚ calculate rate of
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Burette (4) V. Burette Stand (4) VI. Stopwatch VII. Conical flask (2) Reagents I. 0.020M I2 (aq.) II. 2.0M CH3COCH3 (aq.) III. 2.0M HCl (aq.) Raw Data Absorbance of I2 Solution Volume of I2 Solution/ cm3 ± 0.1 Volume of distilled water/ cm3 ± 0.05 [ I2 (aq.)]/ mol dm-3±0.0005 Absorbance ± 0.001 0.0 10.00 0.0000 0.000 1.0 9.00 0.0020 0.097 2.0 8.00 0.0040 0.175 3.0 7.00 0.0060 0.261 4.0 6.00 0.0080 0.356 5.0 5.00 0.0100 0.440 The difference
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