The Activity of a Protease (Trypsin) Introduction Enzymes catalyze reactions by creating alternate reaction mechanisms whose transition states are more thermodynamically stable than uncatalyzed reactions (Berg et al.‚ 2002; UBC Department of Microbiology and Immunology‚ 2006). Increased thermodynamic stability in these transition states reduces the energy of activation‚ the minimum amount of energy input a chemical system requires for a reaction to occur (UBC Department of Microbiology
Premium Enzyme inhibitor Enzyme PH
used to calculate unknown protein concentrations of given samples. The absorbances of the whole milk‚ cereal milk and muscle milk had been previously obtained and recorded via spectrophotometer (Table 2). These absorbances were substituted into the y- value of the equation and the concentrations‚ x-value‚ were solved for and recorded in Table 4. It is important to note that some of the samples required dilutions when their absorbance
Premium Protein Chemistry
and spectrophotometric methods were used to determine the specificity of invertase by determining the amount of glucose converted from the given disaccharides. The results show that sucrose yielded the least amount of glucose and got the lowest absorbance reading. INTRODUCTION Enzymes are globular proteins. Their folded conformation creates an area known as the active site. The nature and arrangement of amino acids in the active site make it specific for only one type of substrate. Enzymes
Premium Enzyme Sucrose
reaction between crystal violet and sodium hydroxide reacts appropriately‚ then the order will be first order. Procedure: Mix 10 mL of sodium hydroxide and crystal violet solution together. After calibrating the colorimeter‚ insert the mixture. Absorbance data will be collected for three minutes. Analyze the data graphically to determine the reaction. Discussion: The hypothesis that I stated was sadly‚ not supported by the data that my partner and I obtained. Comparing the regression of the two
Premium Chemistry Rate equation
Title: Confirming the Presence of Fermentation in the Conversion of Milk to Kefir Authors: Michael Ahrens‚ Nicholas Fiore‚ Garrett Hages‚ Melissa Cullom University of Kansas‚ Biol 402‚ Fall 2014‚ 3:00pm room 6040 Abstract: In this experiment milk was fermented into Kefir‚ then a series of tests were used to confirm that fermentation actually occurred. The tests used to confirm the fermentation were a gas production test‚ pH test‚ Gram stain‚ and turbidity test. The results showed during the conversion
Premium Carbon dioxide Kefir Gram staining
amount of damage inflicted upon a beet sample. 5. Wavelength used: 470nm 6. We used this wavelength because we were measuring the amount of lycopene in each solution; 470nm is the peak of lycopene’s absorbance. 7. Results table: NaCl Solution Absorbance Reading Class Averages for Absorbance Reading 1 0 % 0.098 0.124 2 3 % 0.127 0.117 3 6 % 0.112 0.126 4 9 % 0.091 0.090 5 12 % 0.108 0.121 6 15 % 0.068 0.117 8. Currently‚ this is a very rough set of data‚ as the individual
Free Concentration Solution Chemistry
Cesar Bizarroque Wesam Daker Bio 1107k-11 10/25/14 How pH levels impact tyrosinase activity Abstract: The purpose of our research utilizing the different pH levels was to test how a specific pH level would impact tyrosinase activity. First we added 4.0 mL of pH in each corresponding test tube and then added 0.5 mL of substrate (catechol) into each test tube. In the instructions it says to apply your 0.5 mL of tyrosinase (potato extract) as well but you have to blank the spectrophotometer before
Premium Enzyme Acid Base
volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic 20 at a wavelength of 540 nm is used to measure the absorbance of protein at different concentrations of the liver extract. The results for this showed that an absorbance of 540 nm‚ as the amount of liver extract increases‚ the absorbance increases. Introduction The main objective of this lab was to measure the amount of protein from a piece of beef liver‚ and then use the spectronic 20
Premium
Chem-102 Lab Dr. Sobhi Experiment #3: Spectrophotometric Determination of Tartrazine Purpose The objectives of this lab include- illustrating the use of the spectrophotometer in chemical analysis‚ and generating a standard‚ or calibration curve‚ then using that curve to determine the value of an unknown substance. The spectrophotometer is one of the most powerful tools used in chemistry to find the concentration of substances in solution. It compares the colors of a known and an unknown solution
Premium Chemistry Solution Spectroscopy
precipitate has formed‚ there are fewer copper ions remaining in the Benedict’s Reagent‚ therefore‚ the solution will appear less blue. This can be measured using a colorimeter‚ as it is known that the concentration of a substance is proportional to its absorbance of light. Fructose is the sugar found in fruit. It is a monosaccaride‚ and is a reducing sugar because it acts as a reductant in chemical reactions. This can be seen in the Benedict’s test where the fructose in the fruit juice will reduce CuSO4
Premium Fructose Citrus Sugar