Biochemistry of Proteins; Isolation of Ovalbumin and Enumeration of thiol groups Abstract Thiol groups are important to protein folding and forming disulphide bonds that are essential to protein structure. Determining the number of thiol groups in a protein is important in determining the tertiary structure of the protein. The ovalbumin is the experiment was purified from egg white using centrifugation and ammonium sulphate precipitation and then the thiol groups identified using DTNB and spectroscopy
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Publication No. 11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity
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follow the growth of Serratia marcescens in nutrient broth at 37oCby recording the changes in turbidity (cloudiness) by measuring the absorbance of visible light (600 nm) and also to prove that there is an increase in the cell number and not just in mass during the growth. In the experiment we measure the full growth curve of Serratia marcescens by measuring the absorbance at 600nm at every 10 mins. I also determined the viable count at the start and the end of the exponential phase of growth. Using
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different rpm to tailor the thickness. The structures of the films are investigated through XRD measurements. Refractive index‚ extinction coefficient and thickness of the films are determined from Variable Angle Spectroscopic Ellipsometery. The absorbance and transmittance is recorded using UV-Vis spectrophotometer. The impact of post deposition calcinations at 500 °C on the structural‚ optical properties of the films & photocatalysis of Nb2O5 is also studied. The effects of thickness on refractive
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University of Nebraska at Omaha Bomb Calorimetry Physical Chemistry 3354 Enthalpy of Combustion: 1‚2-diphenylethane January 6‚ 2014 Author: Jon D. Paul Signature Professor: Dr. Edmund Tisko Date Abstract Experimentation involving constant volume calorimetry produces the heat of reaction for many substances. When choosing adiabatic conditions we are allowed to focus on the reaction system while neglecting everything else. The reactions that are studied should proceed relatively
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with carbon. The objective of this experiment is determining the manganese amount in the steel compound by using UNICAM UV-VIS Spectrometer. Theory: Many molecules absorb ultraviolet or visible light. The absorbance of a solution increases as attenuation of the beam increases. Absorbance is directly proportional to the path length‚ b‚ and the concentration‚ c‚ of the absorbing species. Beer’s Law states that A = ebc‚ where e is a constant of proportionality‚ called the absorptivity. Different
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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ELISA (Enzyme-Linked ImmunoSorbant Assay) ELISA is abbreviated term for Enzyme-Linked ImmunoSorbant Assay. This procedure is one of the most widely used methods in clinical immunology assays to detect the presence and absence of certain antigens or antibodies and also to quantify them when necessary. Quantification can be done in a range of microgram (µg) to nanogram (ng). The ELISA procedure takes advantage of the fact that most proteins will bind firmly to the surface of different kinds
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concentration of Fe(II) in the stock solution. grams of Ferrous ammonium sulfate *1392.14gm*55.851gm*1000mg1g*10.500L=ppm 0.1756 *1392.14*55.851*10001*10.500=50.019 2. Report concentrations and absorbance of standards and samples. Solution # | Stock volume‚ mL | Conc. Fe(II)‚ ppm | Absorbance at 510 nm | | | | 1 | 2 | 3 | Average | 1 | 1 | 0.500 | 0.082 | 0.083 | 0.082 | 0.082 | 2 | 2 | 1.00 | 0.182 | 0.181 | 0.182 | 0.182 | 3 | 5 | 2.50 | 0.476 | 0.476 | 0.479 | 0.477 |
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The total protein content was estimated as proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2‚ 0.4‚ 0.6‚ 0.8‚ 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes‚ 0.1 and 0.2 ml of the extract were taken. In each test tube‚ the volume was made up to 1 ml‚ followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left
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