2013 Abstract: In this article‚ we will experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs. Introduction: Enzymes
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the precision of the method. Table 6.2: Intra-day precision for determination of PHE PHE µg/ml Absorbance at 252.2 nm 1 2 3 Mean %RSD 5 0.011 0.011 0.011 0.011 1.931 10 0.024 0.024 0.024 0.024 1.211 15 0.035 0.035 0.036 0.035 1.428 20 0.045 0.046 0.047 0.046 1.672 25 0.057 0.058 0.059 0.058 1.724 Average % RSD 1.215 Table 6.3: Intra-day precision for determination of EBS EBS µg/ml Absorbance at 274.8 nm 1 2 3 Mean %RSD 5 0.052 0.051 0.053 0.052 1.923 10 0.099 0.101 0.103 0.101 1.980 15 0
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structural characteristic of DNA molecules through UV spectrum and thermal denaturation and to understand how pH and ionic strength affect the stability of DNA which lead to the shift of melting temperature Tm. Hyerchromic effect is the increase of absorbance at 260nm during the double strand DNA is unwounded into two single strands DNA during denature. Q2. Construct melting curves of DNA at different conditions on the same graph. (2 marks) Q3. Define the term Tm. Describe the melting curve
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pre-filtered dye reagent was added to each tube and vortexed for additional 10 seconds‚ followed by an incubation period of 10 minutes (Course Supplement for Bio 101‚ p. 71). We transferred 500 uL of the solution to the cuvette and measured the absorbance to 595nm. Using the BSA stock solution (2mg/mL=2000 ug/mL)‚ we prepared the concentration of the protein standard by carrying out serial dilution at 2x‚ 5x‚ 10x‚ 20x and 40x. The 2x dilution was made by mixing 250 uL of BSA and 250 of the homogenizing
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EXPERIMENT 2 : UV-VISIBLE DETERMINATION OF AN UNKNOWN CONCENTRATION OF KMnO4 OBJECTIVE 1. To determine the maximum wavelength of potassium permanganate. 2. To plot the calibration curve of potassium permanganate. 3. To determine the concentration of an unknown solution of potassium permanganate. APPARATUS Beaker‚ burette‚ glass rod ‚volumetric flask 100ml ‚dropper CHEMICALS Potassium permanganate (KMnO4)‚ distilled water PROCEDURE A. Preparation of the KMnO4 standard solution
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iron(II) standards. Introduction Fe3+ in a dietary tablet is converted to Fe2+ and reacats with 1‚10-phenathronile to form the tris(1‚10-phenanthroline) iron (III) complex. Hydroquinone is used as the reducing agent ro reduce Fe3+ to Fe2+. The absorbance of the complex is measured using the UV-Visible spectrometry. In atomic absorption atoms absorb part of the light from the source and the remainder of the light reaches the detectors. Atomic emission comes from atoms that are
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The Effects of Various Chemicals and Temperature on Membrane Permeability of Beetroot Name: Ghazal Daneshfar E-mail: GDANES200@caledonian.ac.uk Student ID: S1312108 INTRODUCTION The cell membrane consists of mostly phospholipids and proteins which gives the cell its selectively permeable nature. The function and permeability of the cell membrane depends on its whole structure. When destroyed‚ the permeability of the cell membrane is disrupted causing cellular contents to leak out
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plotting ln [A]/[B] against time (find slope of line where b=2 and a=1). EDTA in this experiment is used as a masking agent to hide metal ions that would normally interfere with the analysis in this reaction. Thus the absorbance of [Fe(CN)6]3- at time t is given by: (6) Absorbance = 1012 [Fe(CN)6]3- The oxidation of C6H8O6 by [Fe(CN)6]3- involves a mechanism that
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Absorption Spectra of Conjugated Dyes 04-21-2103 Introduction: The visible absorption bands or conjugated dye arise from electron transitions involving the electrons in the conjugated and they are free to move along the chain and are not attached to any atom. An example of such a dye is 3‚3-diethyl-thiacyanine iodide. The cation has two resonance forms causing each of the bonds in the conjugated chain to have an order of 1.5 and have a length similar to the C--‐C bond length in benzene
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Title: Isolation and purification of bovine milk α-lactalbumin. Abstract: The Bradford assay‚ a colorimetric protein assay‚ is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250 in which under acidic conditions the red form of the dye is converted into its bluer form to bind to the protein being assayed. The (bound) form of the dye has an absorption spectrum maximum historically held to be at 595 nm. Objective: To determine the technique to a series of protein purification
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