Based on the data from the riboflavin spectrum scan‚ the maximum absorbance wavelength for this compound is 446 nm. This was the point between 390 nm and 500 nm at which the absorbance value (0.72) was the highest. A blank tube that has the components of the solution being examined except for the compound of interest is then used in combination to provide an even more accurate reading. Then‚ by using Beer’s Law‚ the molar extinction coefficient for riboflavin was able to be calculated: 14‚400 L/(moles*cm)
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5 ml sodium carbonate. Then put it in the sec 20 to determine the absorbance of each solution. The concentration enzyme was obtained by with the standard curve as attached. The rate of reaction is calculated by concentration/time. * The dependent variable is the rate of reaction. The independent variable is pH levels. The controlled variable is temperature in the room and the enzyme concentration. Enzyme pHLevel | Absorbance | ProductConcentration | Rate | pH7 | 1.134 | 312.89 | 1043 nmol/min
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distance‚ followed by chlorophyll a. Chlorophyll b is the most polar; therefore‚ it travels the shortest distance. The separated pigments on the chromatography paper can be eluted in acetone and absorbance spectrum is determined using spectrophotometer. Spectrophotometer produces a graph of the absorbance spectrum which shows the wavelengths (in nm) that are absorbed by the pigment and that are reflected. The reflected wavelength of chlorophylls a and b reflect the wavelengths of l light that are
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Jay A. Torres With David Shbeeb and Matt Shinsky Liberty University 1003 Misty Mountain road apt.507‚ V.A. 24502 jtorres99@liberty.edu words count: 1‚313 Determination of Km and Vmax of Alkaline Phosphatase Graphs will be used with the enzyme alkaline phosphatase of the unknown in the enzyme solution to Determine Km and Vmax To Dr. Kimberly A. P. Mitchell‚ Editor‚ Liberty Journal of Cell Biology‚ 1971 University Blvd‚ Lynchburg‚ VA 24502. Materials and Methods Dilution: Alkaline Phosphatase
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The Kinetics of α-Chymotrypsin Introduction Chymotrypsin is a protease which cleaves proteins by a hydrolysis reaction‚ it does this by adding a molecule of water to a peptide bond. Although the hydrolysis reaction is thermodynamically favoured in the absence of a catalyst the half-life for a typical hydrolysis reaction by a protease is between 10 and 100 years‚ needless to say it is extremely slow1. Though this is true peptide bonds are hydrolysed within milliseconds in the body in the presence
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is dissolved in sulphuric acid to create the blue solution of copper. We put the blue solution of copper in the colorimeter and measured the absorbance of the solution 3 times then we found the average of the 3 different results we had gotten. We then diluted the blue solution of copper with water and put it into the colorimeter and measured the absorbance 3 times again and got the average again. A colorimeter is a light-sensitive instrument that measures how much colour is absorbed by an object
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GROUP MEMBERS: VIMAL SIEWNARINE - #52844 JASON MATHURA - #60927 FIDEL MENDOZA- #56834 BRAD NANDO- # VIMAL BALAY - #52555 CRISTINA LUTCHMAN -#52516 LAB #1‚ #2‚ #3‚ #4 CHEM 2006 -ANALYTICAL INSTRUMENTATION LECTURER – MRS. TRICIA JONES LAB 1 TITLE: Organic Compound Identification Using Infrared Spectroscopy Aim: To identify the functional groups in organic compounds using infrared spectra. APPARATUS AND REAGENTS: Nicolet 380FTIR
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of blue food coloring solution and 3.2 mL of NaOCl were mixed and placed in the Spectronic 20. The transmittance of the mixture was recorded at one minute intervals. By using Eq. 1‚ these values were converted to obtain the absorbance of the blue food coloring. The absorbance was then used to determine the concentration of the blue food coloring by using the following
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passing through the solution. * Beer’s Law says that there is a logarithmic relationship between the transmittance and the absorbance of a solution. The absorbance value of the samples can be calculated from the measured transmittance values using Beer’s Law. Then the absorbance values would be used to find the equilibrium constant Kc of the reaction. The absorbance of a solution is directly proportional to its concentration. Procedure The spectrophotometer was first warmed up for fifteen minutes
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➢ Stopclock ➢ Test tubes ➢ Small measuring cylinders ➢ Cuvettes ➢ Cork borer ➢ Mounted needles ➢ Beaker about 250 cm³ ➢ Blue Filter The dependent variable was the absorbance. We measured it by using a colorimeter which compares the amount of light getting through a solution with the amount which can get through a sample of pure solvent. It was controlled by filling the test tubes with distilled water. The
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