pH and Chromatography Lab Report Kevin Rivera Biology Mr. Langley 2C 10/7/13 Introduction In this lab‚ of pH and Chromatography‚ in the pH aspect of the lab we are trying to figure out the pH level of certain chemicals by writing down of known solutions to find the type of unkown solution using pH standards. In the Chromatography‚ we are trying to separate the chemicals using water and a piece of paper. Methodology Materials: Safety Goggles pH indicator & pH indicator key
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CLINICAL CHEMISTRY – defined as the study of biochemical processes associated with health & dse & the measurement of constituents in body fluids or tissues to facilitate dx of dse - dual nature since it’s both a basic and applied science thus clin chem. technologists & technicians should have an understanding of the physiologic & biochemical processes occurring in the body‚ as well as technical skills to perform the various tests - chemistry in connection with the management
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notes on the relationship between the structure‚ properties and functions of glycogen and cellulose. 10) Write notes on the 3 main types of column chromatography (not HPLC)‚ explaining their mode of action and principal uses. 11) Write notes on TWO of the following chromatographic techniques: Thin layer chromatography‚ gel (size exclusion) chromatography‚ ion exchange
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First‚ the amount of total protein removed by the Affinity Chromatography is difficult to quantify because there appears to be an error in the analysis data for 65% cut precipitate. The protein from the previous purification step limits the amount of protein that can be recovered by the subsequent purification step. In this case‚ the total protein recovered by 65% cut was 159 mg‚ but Affinity Chromatography recovered 350 mg of protein. Purification steps cannot gain protein unless
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Experiment # 4 Paper Chromatography 1. Why is the chromatogram developed in an essentially closed system? - The chromatogram is developed in a closed system in order to prevent the solvent to evaporate. Most solvents used in the chromatograph are toxic and flammable. It is also put in a close system to reduce the chance of outside factors affect the chromatograph. 2. What is the main advantage of 2-dimensional paper chromatography over a 1-dimensional one? - The
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salt sugar An experiment is carried out to separate a mixture of two dyes. A line is drawn on a piece of chromatography paper and a spot of the dye mixture placed on it. The paper is dipped into a solvent and left for several minutes. paper spot of mixture of dyes beaker solvent level solvent line Which statement about this experiment is correct? A B C D 3 The dyes must differ in their boiling points. The dyes must differ in their solubilities in the solvent. The line must be drawn
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IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different solubilities and adsorption
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solutions: Lab report: outline Ink chromatography Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. It is used to separate substances. Examples of chromatography are finger-printing‚ medicines‚ and separating/ testing histamines. http://www.biotecharticles.com/Agriculture-Article/Applications-of-Chromatography-1299.html The purpose of this lab
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Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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