will cut and the number and size of DNA pieces that will result. These pieces can then be separated and compared using a process called gel electrophoresis. The DNA moves from the negative end to the positive end. As the fragments move‚ their varying lengths propel them through an agarose gel at different speeds. Short strands move through the holes in the gel more quickly than long strands and will over time move farther away from the starting point. Staining the sorted groups of DNA makes them
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Systems Biology Systems biology involves the study of an organism as one single system. Instead of analysing all the individual components that make up a cell‚ the cell is instead viewed as an interacting network of genes‚ proteins and biochemical reactions and these are studied as a whole. In 20th century‚ molecular biology was focused upon. A ‘reductionist’ approach was followed‚ in which the individual components‚ such as the cell nucleus or sugar metabolism‚ were studied in isolation. However
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Which type of bone and what are the specific bones that are best for height/stature determination? Why is it best to have two or more bones for height calculations? 1.3.1 Restriction enzymes recognize and cut specific sequences in DNA. Gel electrophoresis separates DNA fragments based on size and is used in Restriction
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Antibiotic Production from the plant Lantana camara Project Summary The purpose of this experimental procedure is targeted to find a novel bacterium from the soil of Lantana camara that can be used in the dental field to kill or stop the growth of the Streptococcus mutans bacteria. The experimental procedure is to take place over a time frame of ten weeks conducted by a student team of four. The proposed methodology to take place is to cultivate and purify isolates from the soil‚ find antibiotic
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Proteins are the most abundant macromolecules found in living organisms because they can perform multiple task in the body. The task proteins are known to carry out are compound transporters‚ structural support in hair and nails‚ protecting against infections and catalyzing reactions. However‚ to study a certain protein it has to go through purification because cells can contain thousands of proteins. Purification allows scientist to know what molecule substrates are present‚ if the enzyme activity
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1 Detecting glowing E.Coli Colonies by making recombinant DNA from the lux operon of Vibrio Fischeri to pGEM. Liao‚ Tffany The marine bacterium Vibrio Fischeri produced bioluminescence effect due to lux operon transcription. The purpose of the experiment is to create a genomic library of Vibrio DNA and clone the lux operon by making Recombinant DNA and transform into another organism‚ E. Coli. Chromosomal DNA of vibrio fischeri was first extracted and digested with restriction enzyme Sal I‚ then
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insertion and finally the (-/-) indicates that neither parent contributed an Alu insertion. Aim The aim of the experiment is to isolate purified genomic DNA from cheek cells‚ prepare the sample for PCR reaction‚ place prepared sample in agarose gel through a procedure called
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In this coursework I will be exploring two issues‚ my major issue being DNA Fingerprinting and my minor issue is PCR (Polymerase Chain Reaction). DNA Fingerprinting (Obtained from www.anselm.edu/.../genbio/geneticsnot.html) (The diagram above shows that the defendant had the victim’s blood on his clothes) Web Description: A method of comparing the genetic similarities or differences between individuals. This technology is often used as a forensic tool to identify the source of blood
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techniques. Agar plates labeled LB‚ LB/AMP‚ and LB/AMP/ARA containing ampicillin (AMP) and arabinose (ARA) were used to grow of the bacteria of interest and SDS-PAGE gels were utilized in identifying the fluorescent and non-fluorescent proteins. The end results illustrated that there were no signs of fluorescent proteins in the gel bands and there were red fluorescing bacteria in the LB/AMP plate that should not have been. The agars contained in all three plates was exposed to arabinose‚ and there
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Investigation of the probiotic properties of bacterial strains from two probiotic drinks and their survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating
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