Which type of bone and what are the specific bones that are best for height/stature determination? Why is it best to have two or more bones for height calculations? 1.3.1 Restriction enzymes recognize and cut specific sequences in DNA. Gel electrophoresis separates DNA fragments based on size and is used in Restriction
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Proteins are the most abundant macromolecules found in living organisms because they can perform multiple task in the body. The task proteins are known to carry out are compound transporters‚ structural support in hair and nails‚ protecting against infections and catalyzing reactions. However‚ to study a certain protein it has to go through purification because cells can contain thousands of proteins. Purification allows scientist to know what molecule substrates are present‚ if the enzyme activity
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1 Detecting glowing E.Coli Colonies by making recombinant DNA from the lux operon of Vibrio Fischeri to pGEM. Liao‚ Tffany The marine bacterium Vibrio Fischeri produced bioluminescence effect due to lux operon transcription. The purpose of the experiment is to create a genomic library of Vibrio DNA and clone the lux operon by making Recombinant DNA and transform into another organism‚ E. Coli. Chromosomal DNA of vibrio fischeri was first extracted and digested with restriction enzyme Sal I‚ then
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insertion and finally the (-/-) indicates that neither parent contributed an Alu insertion. Aim The aim of the experiment is to isolate purified genomic DNA from cheek cells‚ prepare the sample for PCR reaction‚ place prepared sample in agarose gel through a procedure called
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techniques. Agar plates labeled LB‚ LB/AMP‚ and LB/AMP/ARA containing ampicillin (AMP) and arabinose (ARA) were used to grow of the bacteria of interest and SDS-PAGE gels were utilized in identifying the fluorescent and non-fluorescent proteins. The end results illustrated that there were no signs of fluorescent proteins in the gel bands and there were red fluorescing bacteria in the LB/AMP plate that should not have been. The agars contained in all three plates was exposed to arabinose‚ and there
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In this coursework I will be exploring two issues‚ my major issue being DNA Fingerprinting and my minor issue is PCR (Polymerase Chain Reaction). DNA Fingerprinting (Obtained from www.anselm.edu/.../genbio/geneticsnot.html) (The diagram above shows that the defendant had the victim’s blood on his clothes) Web Description: A method of comparing the genetic similarities or differences between individuals. This technology is often used as a forensic tool to identify the source of blood
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Investigation of the probiotic properties of bacterial strains from two probiotic drinks and their survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating
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pellet cells with 50µl of 0.7% low melting agarose. The resuspended cells were spread over 0.5% normal melting agarose pre-coated slides. Then‚ the slides were immersed in lysis buffer for at least 1hour prior to deep them in the electrophoresis chamber containing electrophoresis buffer for 20 min‚ followed by another 20min with running currency at 1.25V/cm and 300mA. Comets were stained with ethidium bromide (50µl: 10µg/ml)‚ and were recorded by scoring 150 cells per each animal under fluorescent microscope
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we went on excursions to private laboratories. The most exciting experience was where we aimed to induce over-expression of a particular gene in bacteria. We used the polymerase chain reaction technique‚ the gel electrophoresis with agarose followed by isolation of fragments from the gel‚ bacterial plasmid isolation‚ restriction mapping and during class excursions I got to see the use of this techniques on a large scale like the production of antibiotics using the process of fermentation
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NcoI and XhoI (Fermentas‚ Lithuania) enzymes. The expression vector pET28a was also digested with NcoI and XhoI. The double digestion was carried out at 37ºC‚ digested fragments were analyzed by agarose gel electrophoresis and the products were purified (Fermentas‚ Extraction of DNA from the gel kit‚ Lithuania). The digested products‚ pET28a expression vector and V-Domain fragments‚ both were ligated by T4 DNA ligase‚ with the ration of 1:6 of vector and V-domain gene‚ and incubated first at 16 ºC
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