ratio is considered to be the most significant factor in triggering a cell to divide‚ and therefore‚ determining cell size. OBJECTIVES • Determine the extent and rate of diffusion into three different size agar cubes. • Calculate the surface area to volume ratio for each agar
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ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride ions rather than sodium ions because while the sodium ions do diffuse in to the agar the chloride ions from the NaCl will combine with silver ions‚ from AgNO3 and create a white precipitate. The white precipitate allows us to measure the the white band through the agar at different points and determine the rate of diffusion of the chloride ions. If the the agar concentration was
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antibiotic-producing microbes in the soil that was taken from a local sample. To do so‚ two different samples of bacteria have been taken and placed onto agar plates (labelled A and B). As we allow each sample to grow‚ our objective will be to see what‚ if any‚ antibiotics develop and inhibit the growth of the bacteria. We predict that antibiotics will form on the agar plates‚ thus inhibiting the growth
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experiment. In part A of the lab‚ diffusion was demonstrated with two solids and an agar gel petri dish. One crystal of potassium permanganate and one crystal of methylene blue were placed on either side of an agar gel petri dish. The purpose of this experiment was to determine which of the crystals would diffuse across the gel more. So the question is‚ which solid would have a higher rate of diffusion through the agar‚ methylene blue or potassium permanganate? Diffusion is the movement of particles
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Absract The aim of the prac was to identify and isolate Staphylococcus aureus and Eschericia coli in milk and salmonella in poultry.It was to investigate bacteriological quality of milk and poultry. Salmonella is areprobably the most important cause of food borne illness globally.Staphylocooccus aureus all cause food borne diseases if consumed in a contaminated milk. For milk a spread plate method was used and for poultry a streak method was applied using different Medias. All food contain a certain
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Background: In Jane Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus
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Selective enrichment broth- with Tetrathionate Brilliant Broth Selective Plating- Brilliant Green Agar Isolation of salmonella conformation- is preformed using TSI and LIA Test results Tetrathionate Brilliant Green Broth (TGBG)was inoculated from the whirl pack spinach infected bag. TBGB inhibits the growth of organisms other than salmonella. The next test preformed was the Brilliant Green Agar (BGA)
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aureus. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. The tests used to identify the unknown bacterial culture included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and
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performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample. The tests included: Triple sugar iron agar (TSAI)‚ the Sulfide Indole Mobility (SIM) test‚ Glucose fermentation‚ the Methyl Red test‚ the Voges-Proskauer test‚ Citrate test‚ the Urease Test‚ and finally the Gelatin test. The microbes that were tested during this lab were: Escherichia coli
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