antibiotic-producing microbes in the soil that was taken from a local sample. To do so‚ two different samples of bacteria have been taken and placed onto agar plates (labelled A and B). As we allow each sample to grow‚ our objective will be to see what‚ if any‚ antibiotics develop and inhibit the growth of the bacteria. We predict that antibiotics will form on the agar plates‚ thus inhibiting the growth
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of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride ions rather than sodium ions because while the sodium ions do diffuse in to the agar the chloride ions from the NaCl will combine with silver ions‚ from AgNO3 and create a white precipitate. The white precipitate allows us to measure the the white band through the agar at different points and determine the rate of diffusion of the chloride ions. If the the agar concentration was
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Background: In Jane Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus
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Absract The aim of the prac was to identify and isolate Staphylococcus aureus and Eschericia coli in milk and salmonella in poultry.It was to investigate bacteriological quality of milk and poultry. Salmonella is areprobably the most important cause of food borne illness globally.Staphylocooccus aureus all cause food borne diseases if consumed in a contaminated milk. For milk a spread plate method was used and for poultry a streak method was applied using different Medias. All food contain a certain
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aureus. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. The tests used to identify the unknown bacterial culture included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and
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Selective enrichment broth- with Tetrathionate Brilliant Broth Selective Plating- Brilliant Green Agar Isolation of salmonella conformation- is preformed using TSI and LIA Test results Tetrathionate Brilliant Green Broth (TGBG)was inoculated from the whirl pack spinach infected bag. TBGB inhibits the growth of organisms other than salmonella. The next test preformed was the Brilliant Green Agar (BGA)
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performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample. The tests included: Triple sugar iron agar (TSAI)‚ the Sulfide Indole Mobility (SIM) test‚ Glucose fermentation‚ the Methyl Red test‚ the Voges-Proskauer test‚ Citrate test‚ the Urease Test‚ and finally the Gelatin test. The microbes that were tested during this lab were: Escherichia coli
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3. With a pipet‚ inoculate the agar dish labeled water with the water sample. Use only enough water to cover the top surface of the dish (approximately 4 drops). 4. Cover the dish and let it sit for 30 minutes to ensure the water soaks into the agar. 5. Incubate the dish upside down at room temperature for 24–72 hours. 6. Observe the dish and count the number and types of colonies. Record the results in Data Table 1: Environmental Colony Formation‚ in the Lab Report Assistant section. 7. Soak
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Teeter Foaming Hand Soap is more effective in reducing bacterial growth than the Soft Soap hand soap‚ then there will be less bacterial/microorganism growth present on the agar plate for the Harris Teeter Foaming hand soap. II. Materials and Methods c. Obtain 3 agar plates and 2 different brands of hand soap. Label each agar plate and draw a line to separate each plate into 2 sections (control and experimental). d. Touch control side of each plate with hands rinsed only with water
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A. Describe the difference between an obligate and facultative aerobe. An obligate aerobe is a microorganism that must have oxygen for growth and will grow at the top of the test tube. A facultative aerobe prefers oxygen but it is not necessary for growth. Many of these microorganisms will grow at the top of the tube‚ but will also be dispersed throughout as well. B. Discuss the temperature requirements of the organisms (you grew) in this lab. Both Staphylococcus epidermis and Lactobacillus acidophilus
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