ABSTRACT The effect of molecular weight on the rate of diffusion was measured using two tests namely: the glass tube test and the agar-water test. The set-up of the glass tube test used two cotton balls of the same size. One cotton ball is moistened with hydrochloric acid (HCl) and the other one is moistened with ammonium hydroxide (NH4OH). The two cotton balls were inserted in both ends of the glass tube. NH4OH which has a lighter molecular weight (35.0459 g/mole) diffused with a faster rate (dave=20
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A streak culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed
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Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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(RESEARCH TREATISE) By SITUMBEKO LIWELEYA (s213459531) Submitted in fulfilment of the requirements for the degree of BACHALOROUS TECHNOLOGIEA: BIOMEDICAL TECHNOLOGY At the At Nelson Mandela Metropolitan University Port Elizabeth‚ 2013. SUPERVISOR- PROFESSOR SMITH. N. DECLARATION I‚ the undersigned‚ hereby declare that the research work contained in this study is my own original work‚ and all the sources I have used or quoted have been indicated and acknowledged
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• Eye protection • 250 mL beaker • Timing device • Scoopula • Ruler • Scalpel • Sodium hydroxide solution • 3 different sized cubes of phenolphthalein agar • Paper towels Purpose 1. Put on eye protection 2. With the scalpel cut the block of phenolphthalein agar in to a 1x1x1 cm cube (Cube A)‚ a 2x2x2 cm cube (Cube B) and a 3x3x3 cm cube (Cube C). 3. Pour enough sodium hydroxide solution in to the beaker to cover the
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INTRODUCTION Cassia fistula Linn. (Leguminosae) is a very common plant and is widely known for its medicinal properties. In the Indian literature‚ this plant has been described to be useful against skin diseases‚ liver troubles‚ tuberculous glands and its use in the treatment of rheumatism‚ hematemesis‚ pruritus‚ leucoderma‚ and diabetes.[1‚2] Besides‚ it has been found to exhibit anti-inflammatory and hypoglycemic activity and widely used as a mild laxative suitable for children and pregnant women
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the 2 tests: the glass tube setup and the water agar-gel setup. In the glass tube setup‚ two cotton balls were soaked in the solutions of hydrochloric acid (HCl) and ammonium hydroxide (NH4OH) and were simultaneously placed on both ends of the tubing.NH4OH had a lighter molecular weight of 35 g/mole which diffused at a faster rate of 24.8 cm and formed a white smoke near the HCl end that had the molecular weight of 36 g/mole. The water agar-gel setup was made up of a petri dish containing
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the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH4OH‚ M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm)‚ resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl‚ M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed
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for specific organisms. For i.e. Mackonkey agar only grows on gran(-). If a sample is negative it grows and if it is positive it doesn’t grow. Last but not least Differential media relies on color change of the media itself. This color change highlights the bacteria if it has a specific trait. For i.e. If sugar ferments there will be a color change. III. Reagents: 1.Tryptone 2.Yeast extract 3.NaCl 4.KCl 5.MgCl2 6. MgSO4 7.Glucose/ Dextrose 8.H20 9.Agar( for solid) IV. Methods Grab an Erlenhymer
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General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example of a differential medium. In Simmons Citrate Agar‚ there is a pH indicator that turns from green to blue when citrate is utilized as the sole carbon source
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