microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized‚ a flame sterilized inoculating loop will be used to gather
Premium Bacteria Microbiology Gram staining
semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice and water in varying concentrations (1:10‚ 1:20‚ 1:40‚ 1:80) was added to a nutrient agar plate inoculated with S. epidermis and a nutrient agar plate inoculated with E. coli. The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against either
Premium Bacteria Microbiology Escherichia coli
with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae e.coli (left) k.pneumoniae (right) *TSI ALK/Ag-----Serratia marcecens‚ Salmonella paratyphi A red slant‚ yellow butt with cracks or agar displacement or bubbles if with:
Premium Escherichia coli
Day 1 Throughout the semester‚ I have learned multiple techniques for identifying bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive
Premium Bacteria Microbiology Gram staining
cells growing on soft agar burst from the viral infection and appears like a hole in the agar. Each plaque is created by the progeny of an individual phage and can thus be counted to determine the number of phage particles in a sample. The purpose of this lab is to employ a plaque assay method to determine the number of infected phage particles in the given sample. Method & Materials: * (6) BHI plates at 37˚C * (5) tubes of 9.0mL TSB broth * (6) tubes of soft agar in a 50˚C water bath
Premium Bacteria Escherichia coli
Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
Premium Bacteria Microbiology Microorganism
Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey Agar colonies were used to inoculate Gram-negative tests. Once the colonies were isolated and the appropriate Gram-negative and Gram-positive tests were conducted‚ the identification of both unknown organism were fairly easy. The results from the
Premium Bacteria Microbiology Escherichia coli
purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials : 1) Blood agar plate . 2) Mannitol Salt agar (MSA) plate. 3) DNase agar plate . 4) Novobiocin disc . 5) Inoculating loop. 6) flame ( Bunsen burner) . 7) 1N hydrochloric acid (HCl) . 8) Two slides . 9) Plasma tube. 10) 3% Hydrogen Peroxide (H2O2) . 11) One unknown plate . 12) Crystal
Premium Staphylococcus Bacteria Staphylococcus aureus
behaviors of different cellular slime molds were analyzed under different conditions. They found that in D. disciodeum thrived equally on nutrient soil and a non-nutrient agar dishes (Bonner & Lamont‚ 2005). Similarly to the experiment preformed by Bonner and Lamont‚ we evaluated growth patterns of D. disciodeum on non-nutrient agar dishes. Another study conducted by Fisher‚ found that certain genetic factors increase the movement and development of D. disciodeum amoebae within a temperature gradient
Premium Dictyostelid Slime mold Developmental biology
turn pink PEA prevents gram negatives from growing (2) PEA prevents the growth of Gram-negative organisms by disrupting the structure of lipids in the Gram-negative membrane. It also can hamper protein synthesis. Hemolysis is displayed on blood agar plates (2) On the plate the bacteria produces enzymes called hemolysins these enzymes lyse the red blood cells to certain degrees. Bacteria the fully lyse the blood go through beta-hemolysis. Bacteria that partially lyse the blood go through alpha-hemolysis
Premium Mass media Chemical reaction Chemistry