allows the GFP gene to be expressed‚ but in both cases bacteria colonies would be present because of the gene of resistance to the antibiotic‚ ampicillin). We essentially made the required transformed solutions--and the controls--swiped them on the agar plate‚ and then observed to see whether or not bacteria colonies grew and whether or not they glowed. Our data fully supported our hypothesis. We can thus conclude that bacteria can take in foreign DNA through the process of transformation and that
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Different Molar Mass on the Diffusion on Substances Lunar-maius A. Gaerlan Group 2 Sec. X – 9l August 15‚ 2012 ABSTRACT The effect of molecular weight on the rate of diffusion was assessed using agar-water gel test. The agar-water gel set up was composed of a petri dish of agar-water gel containing three wells. Drops of potassium permanganate (KMnO4)‚ potassium dichromate (K2Cr2O7) and methylene blue(C16H18N3SCl) were simultaneously introduced to each well. Methylene blue‚ having the
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bacterial walls. The solutions and fluids studied were saliva‚ mucus‚ tears‚ a stock solution of lysozomes‚ and distilled water. The solutions were placed in agar containing Micrococcus Luteus and we observed the amount of bacteria that was lyzed around them. The measurements were taken by observing where the agar cleared around the solutions‚ as the agar was cloudy where bacteria was present. I hypothesized that saliva would
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Materials • 22 prepared nutrient agar in petri dishes • Timer • Starburst candy (11) • 22 sterile swab • ½ slice of Bologna- Oscar Myer (11) • Sterile gloves G. Procedures 1) Prepare 4 petri dishes with nutrient agar. 2) Put on your sterile gloves 3) Pick up your first food (Starburst candy) and drop it on the ground. 4) Start the timer. 5) Pick up the Starburst candy from
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Molecular Biology Lab Report Payton Jackson Introduction In this lab‚ I am going to use antibiotic-resistance plasmids to transform Escherichia coli. Materials For this lab you will need the following: LB Agar Petri dishes Beakers Test tubes CaCl2 solution Sensitive E. coli (-ampR) amp plasmids ampicillin -amp cells Water bath to heat shock cells A freezer to incubate cells Process Step 1: Wash hands and sanitize lab setting. This will prevent anything reacting with a
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Identification of Bacterial Pathogens basic skills in diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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synthesis of ATP (Hertfordshire‚ 2011). Without ATP cellular functions fail and then die. Research Question: How does increasing the concentrations of Dettol Antiseptic Liquid from 0% to 100% affect the growth of Escherichia coli bacteria on the agar plates? Hypothesis: As the concentration of Dettol Antiseptic Liquid increases the inhibition of bacterial growth around the antiseptic disks also increases. Variables: |Type of variable
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Medical Mycology: Yeast and Pneumocystis| Reading Assignment:|Mahon‚ Chapter 10‚ pgs 215-219‚ Chapter 27‚ pgs 626-629‚ 634-636‚ Appendix B Lecture Notes: Medical Mycology| |U of W Tutorial on Mycology (organisms listed in objectives)‚ www.medtraining.org[->0]| _____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the
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